- ￥2890
- LSM Bio
- 0.15-10NG/ML
- 0.078NG/ML
- 国产(香港)
- EF007301
- 2026年05月21日
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- 文献和实验
- 技术资料
- 库存：
20
- 供应商：
LSMBIO
- 检测范围：
0.15-10NG/ML
- 检测方法：
夹心法
- 应用：
夹心法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种：
人
- 标记物：
Human Kynurenic acid
- 样本：
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
0.078NG/ML
- 规格：
96T
Kynurenic acid ELISA KIT|Human
Catalog EF007301
Packing 48T/96T
Reactivity Human
Detect Range 0.156-10ng/ml
Kynurenic acid ELISA KIT Sensitivity< 0.094ng/ml
Application Kynurenic acid ELISA KIT|Human is for detecting the concentration of Human Kynurenic acid in serum, plasma, tissue homogenates and other biological fluids.
Storage condition 6 month at 4 Centigrade
Principle of Kynurenic acid ELISA KIT|Human
The microtiter plate provided in Kynurenic acid ELISA KIT|Human has been pre-coated with an Human Kynurenic acid antibody specific to Human Kynurenic acid. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated Human Kynurenic acidantibody preparation specific for Human Kynurenic acid then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human Kynurenic acid, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm . The concentration of Human Kynurenic acidin the samples is then determined by comparing the O.D. of the samples to the standard curve.
*Please noted that Kynurenic acid ELISA KIT|Human is only for RUO (Research Use Only), Not for the diagnostic Purpose!
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文献和实验Human Xanthine Oxidoreductase Determination by a Competitive ELISA
Xanthine oxidoreductase (XO) catalyses the oxidation of hypoxanthine to xanthine and of the latter to uric acid. The enzyme is present in traces in most of human tissues, including plasma, being more abundant in milk, liver, and intestine (1).
调至 1μg/μl。 4. ELISA 检测：按试剂盒说明书完成板平衡、加样孵育、洗板、酶标抗体孵育、显色终止与读数，结合标准曲线得出 IFN-γ 浓度。 四、操作要点速记 1.组织样本新鲜处理，全程低温操作，裂解液现配现用； 2. 蛋白定量时标准品与样本同步孵育，稀释采用倍比稀释减少误差； 3. ELISA 实验需每次重绘标准曲线，洗板充分、严格把控显色与终止时间。 免疫检测试剂盒： 货号-规格 名称 abs510001-96T Human IL-2 ELISA Kit (Faster
Methods for the Detection of D-Amino-Acid Oxidase
al. (5 ) using bovine serum albumin as a standard. It was also determined using a Protein Assay Kit (Bio-Rad, Hercules, CA). D-Amino-acid oxidase activity is finally expressed as the amount of D-alanine oxidized per min per milligram of protein. Hog
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