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- 文献和实验
- 技术资料
- 库存:
20
- 供应商:
LSMBIO
- 检测范围:
0.15-10NG/ML
- 检测方法:
夹心法
- 应用:
夹心法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种:
人
- 标记物:
Human 27 OHC
- 样本:
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
0.078NG/ML
- 规格:
96T
27 OHC ELISA KIT|Human
Catalog EF007285
Packing 48T/96T
Reactivity Human
Detect Range 15.625-1000ng/ml
27 OHC ELISA KIT Sensitivity< 9.375ng/ml
Application 27 OHC ELISA KIT|Human is for detecting the concentration of Human 27 OHC in serum, plasma, tissue homogenates and other biological fluids.
Storage condition 6 month at 4 Centigrade
Principle of 27 OHC ELISA KIT|Human
The microtiter plate provided in 27 OHC ELISA KIT|Human has been pre-coated with an Human 27 OHC antibody specific to Human 27 OHC. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated Human 27 OHCantibody preparation specific for Human 27 OHC then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human 27 OHC, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm . The concentration of Human 27 OHCin the samples is then determined by comparing the O.D. of the samples to the standard curve.
*Please noted that 27 OHC ELISA KIT|Human is only for RUO (Research Use Only), Not for the diagnostic Purpose!
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文献和实验调至 1μg/μl。 4. ELISA 检测:按试剂盒说明书完成板平衡、加样孵育、洗板、酶标抗体孵育、显色终止与读数,结合标准曲线得出 IFN-γ 浓度。 四、操作要点速记 1.组织样本新鲜处理,全程低温操作,裂解液现配现用; 2. 蛋白定量时标准品与样本同步孵育,稀释采用倍比稀释减少误差; 3. ELISA 实验需每次重绘标准曲线,洗板充分、严格把控显色与终止时间。 免疫检测试剂盒: 货号-规格 名称 abs510001-96T Human IL-2 ELISA Kit (Faster
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