- ￥2890
- LSM Bio
- 0.15-10NG/ML
- 0.078NG/ML
- 进口
- EF007001
- 2025年07月10日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSMBIO
- 检测范围：
0.15-10NG/ML
- 检测方法：
夹心法
- 应用：
夹心法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种：
人
- 标记物：
Human MYH10
- 样本：
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
0.078NG/ML
- 规格：
96T
MYH10 ELISA KIT|Human
Catalog EF007001
Packing 48T/96T
Reactivity Human
Detect Range 0.156-10ng/ml
MYH10 ELISA KIT Sensitivity< 0.094ng/ml
Application MYH10 ELISA KIT|Human is for detecting the concentration of Human MYH10 in serum, plasma, tissue homogenates and other biological fluids.
Storage condition 6 month at 4 Centigrade
Principle of MYH10 ELISA KIT|Human
The microtiter plate provided in MYH10 ELISA KIT|Human has been pre-coated with an Human MYH10 antibody specific to Human MYH10 . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated Human MYH10 antibody preparation specific for Human MYH10 then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human MYH10 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm . The concentration of Human MYH10 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
*Please noted that MYH10 ELISA KIT|Human is only for RUO (Research Use Only), Not for the diagnostic Purpose!
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文献和实验QIAGEN Plasmid plus Kit——超快纯化多至10 mg转染级别纯质粒
♦利用真空方式超快速进行质粒纯化，碱裂解步骤后，利用QIAfilter无须离心即可澄清裂解液，然后离心柱安置于真空底座（例如：QIAvac 24 Plus 见图1）上进行DNA的洗涤和洗脱。Midi and Maxi kit 20 分钟内可纯化24 个样本Mega和Giga Kit可分别在40，50分钟内处理12个样本。 ♦QIAGEN Plasmid plus Midi/Maxi Kit 提供优化的高产量protocol 并采取高洗脱体积，确保纯化得到高产量质粒
in the same volume of Buffer 1 as the initial volume transferred from the vial in step 2. 2. Sample Preparation Preparation of MNC from Buffy Coat to Obtain Low Platelet Numbers 1) Dilute 10 – 18 ml buffy coat with Buffer 2 to a total
RNA was purified using the AllPrep DNA/RNA 96 Kit and analyzed on the Agilent 2100 bioanalyzer. Intact RNA is indicated by a RIN value of close to 10. RNA with no genomic DNA contamination. Total RNA was purified from rat kidney
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