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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSMBIO
- 检测范围:
0.15-10NG/ML
- 检测方法:
夹心法
- 应用:
夹心法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种:
人
- 标记物:
Human LDL
- 样本:
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
0.078NG/ML
- 规格:
96T
LDL ELISA KIT|Human
Catalog EF006951
Packing 48T/96T
Reactivity Human
Detect Range 7.813-500ng/ml
LDL ELISA KIT Sensitivity< 4.688ng/ml
Application LDL ELISA KIT|Human is for detecting the concentration of Human LDL in serum, plasma, tissue homogenates and other biological fluids.
Storage condition 6 month at 4 Centigrade
Principle of LDL ELISA KIT|Human
The microtiter plate provided in LDL ELISA KIT|Human has been pre-coated with an Human LDL antibody specific to Human LDL . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated Human LDL antibody preparation specific for Human LDL then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human LDL , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm . The concentration of Human LDL in the samples is then determined by comparing the O.D. of the samples to the standard curve.
*Please noted that LDL ELISA KIT|Human is only for RUO (Research Use Only), Not for the diagnostic Purpose!
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文献和实验Warnings And Limitations This product is for research use only. Not intended for any animal or human therapeutic or diagnostic use unless otherwise stated. Follow appropriate laboratory guidelines. This product contains 0.02% sodium azide
Isolation of LDL(Low Density Lipoprotein) From Human Plasma:
This procedure involves use of human plasma, a potentially dangerous source of blood-borne disease. Wear long-cuffed gloves, and eye-protection. Day one: -Human Plasma is purchased from the Red-Cross. It should be 3-4 days old
RAT/MOUSE GROWTH HORMONE ELISA KIT
实验原理 This assay is a Sandwich ELISA based, sequentially, on: 1) capture of rat or mouse Growth Hormone molecules from samples to the wells of a microtiter plate coated by a pre-titered amount of anti-Growth Hormone
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