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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSMBIO
- 检测范围:
0.31-20NG/ML
- 检测方法:
夹心法
- 应用:
夹心法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种:
人
- 标记物:
Human COL15
- 样本:
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
0.1NG/ML
- 规格:
96T
COL15 ELISA KIT|Human
Catalog EF006714
Packing 48T/96T
Reactivity Human
Detect Range 0.313-20ng/ml
COL15 ELISA KIT Sensitivity< 0.188ng/ml
Application COL15 ELISA KIT|Human is for detecting the concentration of Human COL15 in serum, plasma, tissue homogenates and other biological fluids.
Storage condition 6 month at 4 Centigrade
Principle of COL15 ELISA KIT|Human
The microtiter plate provided in COL15 ELISA KIT|Human has been pre-coated with an Human COL15 antibody specific to Human COL15 . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated Human COL15 antibody preparation specific for Human COL15 then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human COL15 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm . The concentration of Human COL15 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
*Please noted that COL15 ELISA KIT|Human is only for RUO (Research Use Only), Not for the diagnostic Purpose!
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文献和实验samples, MPC-15 for 1–15 ml samples and MPC-50 for 15–50 ml samples. Mixer allowing both tilting and rotation. Buffer 1: PBS (without Ca2 and Mg2 ) w/0.1% BSA and 2 mM EDTA, pH 7.4. Buffer 2: PBS pH 7.4 (without Ca2 and Mg2 ).
A simple workflow allows the purification of high-quality DNA and RNA from the same sample (see flowchart). The 96-well purification plates of the kit are rapidly and conveniently processed using either a centrifuge (Centrifuge 4-15C and Plate Rotor 2 x 96
RAT/MOUSE GROWTH HORMONE ELISA KIT
实验原理 This assay is a Sandwich ELISA based, sequentially, on: 1) capture of rat or mouse Growth Hormone molecules from samples to the wells of a microtiter plate coated by a pre-titered amount of anti-Growth Hormone
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