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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
LSMBIO
- 检测范围:
0.31-20NG/ML
- 检测方法:
夹心法
- 应用:
夹心法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种:
人
- 标记物:
Human SEMA3A
- 样本:
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
0.1NG/ML
- 规格:
96T
SEMA3A ELISA KIT|Human
Catalog EF006264
Packing 48T/96T
Reactivity Human
Detect Range 0.156-10ng/ml
SEMA3A ELISA KIT Sensitivity< 0.094ng/ml
Application SEMA3A ELISA KIT|Human is for detecting the concentration of Human SEMA3A in serum, plasma, tissue homogenates and other biological fluids.
Storage condition 6 month at 4 Centigrade
Principle of SEMA3A ELISA KIT|Human
The microtiter plate provided in SEMA3A ELISA KIT|Human has been pre-coated with an Human SEMA3A antibody specific to Human SEMA3A. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated Human SEMA3Aantibody preparation specific for Human SEMA3A then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human SEMA3A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm . The concentration of Human SEMA3Ain the samples is then determined by comparing the O.D. of the samples to the standard curve.
*Please noted that SEMA3A ELISA KIT|Human is only for RUO (Research Use Only), Not for the diagnostic Purpose!
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文献和实验上海西唐生物科技有限公司 021-55229872, 65333639 www.westang.com 大鼠 Sema3A(Sema3A)ELISA 试剂盒 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗大鼠 Sema3A 单抗包被于酶标板上,标准品和样品中的 Sema3A 与单抗结合,加入生物素化的抗大鼠 Sema3A ,形成免疫复合物连接在板上,辣根过氧化物酶标记的 Streptavidin 与生物素结合
Warnings And Limitations This product is for research use only. Not intended for any animal or human therapeutic or diagnostic use unless otherwise stated. Follow appropriate laboratory guidelines. This product contains 0.02% sodium azide
RAT/MOUSE GROWTH HORMONE ELISA KIT
实验原理 This assay is a Sandwich ELISA based, sequentially, on: 1) capture of rat or mouse Growth Hormone molecules from samples to the wells of a microtiter plate coated by a pre-titered amount of anti-Growth Hormone
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