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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSMBIO
- 检测范围:
0.15-10NG/ML
- 检测方法:
夹心法
- 应用:
夹心法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种:
人
- 标记物:
Hunan DBP/Vitamin D Binding Protein
- 样本:
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
0.078NG/ML
- 规格:
96T
DBP/Vitamin D Binding Protein ELISA KIT|Human
Catalog EF001437
Packing 48T/96T
Reactivity Human
Detect Range 3.906-250ng/ml
DBP/Vitamin D Binding Protein ELISA KIT Sensitivity< 2.344ng/ml
Application DBP/Vitamin D Binding Protein ELISA KIT|Human is for detecting the concentration of Human DBP/Vitamin D Binding Protein in serum, plasma, tissue homogenates and other biological fluids.
Storage condition 6 month at 4 Centigrade
Principle of DBP/Vitamin D Binding Protein ELISA KIT|Human
The microtiter plate provided in DBP/Vitamin D Binding Protein ELISA KIT|Human has been pre-coated with an Human DBP/Vitamin D Binding Protein antibody specific to Human DBP/Vitamin D Binding Protein. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated Human DBP/Vitamin D Binding Proteinantibody preparation specific for Human DBP/Vitamin D Binding Protein then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human DBP/Vitamin D Binding Protein, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm . The concentration of Human DBP/Vitamin D Binding Proteinin the samples is then determined by comparing the O.D. of the samples to the standard curve.
*Please noted that DBP/Vitamin D Binding Protein ELISA KIT|Human is only for RUO (Research Use Only), Not for the diagnostic Purpose!
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文献和实验STAINING NONFILAMENTOUS ACTIN WITH VITAMIN-D BINDING PROTEIN
STAINING NONFILAMENTOUS ACTIN WITH VITAMIN-D BINDING PROTEIN Indirect Method Materials 1. Gc-globulin (vitamin-D binding protein; Calbiochem 345802), prepare 2 mg/ml stock in PBS solution A, clarify 25,000 rpm for 20 min in a 42
ChIP‐chip for Genome‐Wide Analysis of Protein Binding in Mammalian Cells
., Botstein, D., Snyder. M., and Brown, P.O. 2001. Genomic binding sites of the yeast cell‐cycle transcription factors SBF and MBF. Nature 409:533‐538. Ji, X., Li, W., Song, J., Wei
our extensive experience with B-PER for isolating fusion proteins in which the fusion partner is either IPIII-His (an internal protein of bacteriophage T4 with a His tag at the C-terminus)or MBP (the E.coli maltose binding protein).The two cases illustrate
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