- ￥2890
- LSM Bio
- 0.31-20NG/ML
- 0.1NG/ML
- 进口
- ELI-0859h
- 2025年07月11日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSMBIO
- 检测范围：
0.31-20NG/ML
- 检测方法：
夹心法
- 应用：
夹心法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种：
人
- 标记物：
Human anti Glutamic Acid Decarboxylase,anti-GAD
- 样本：
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
0.1NG/ML
- 规格：
96T
Human anti Glutamic Acid Decarboxylase, anti-GAD ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
Glutamic Acid Decarboxylase Autoantibody,GAD-Ab, Anti-GAD,anti Glutamic Acid Decarboxylase,Anti GAD,anti Glutamate decarboxylase,
Search name
Human Glutamic Acid Decarboxylase Autoantibody ELISA KIT ,Human GAD-Ab ELISA KIT ,Human Anti-GAD ELISA KIT ,Human anti Glutamic Acid Decarboxylase ELISA KIT ,Human Anti GAD ELISA KIT ,Human anti Glutamate decarboxylase ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of human Glutamic Acid Decarboxylase Autoantibody,GAD-Ab concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antigen specific to GAD-Ab. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antigen preparation specific for GAD-Ab and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain GAD-Ab, biotin-conjugated antigen and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of GAD-Ab in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验Methods for the Detection of D-Amino-Acid Oxidase
al. (5 ) using bovine serum albumin as a standard. It was also determined using a Protein Assay Kit (Bio-Rad, Hercules, CA). D-Amino-acid oxidase activity is finally expressed as the amount of D-alanine oxidized per min per milligram of protein. Hog
; New York, New York Clinical Monograph Series #2: "Autoantibodies to Glutamic Acid Decarboxylase: Clinical Utility for the Practicing Physician" by R C. McEvoy, M.D., Department of Pediatrics, Division of Diabetes and Endocrinology; Mount Sinai School
一篇文献中的ELISA方法如下：Control peptides (14–17 amino acid sequence near the N-terminal or C-terminal of the studied protein) and the reactive rabbit anti-defensin antibodies (affinity pure) were obtained from Alpha Diagnostics .CoStar flat-bottom high
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