Human 8-Isoprostane ELISA kit

Human 8-Isoprostane ELISA kit

96 Tests

Operating instructions

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

8-epi-prostaglandin F2alpha(8-epi-PGF2α) ,8-Isoprostane

Search name

Human 8-Isoprostane ELISA kit,Human 8-epi-PGF2α ELISA Kit, Human 8-epi-prostaglandin F2alpha ELISA Kit, Human 8-epi-PGF2 alpha ELISA Kit

Intended use

This immunoassay kit allows for the in vitro quantitative determination of human 8-epi-prostaglandin F2alpha,8-epi-PGF2α concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.

Test principle
The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to 8-epi-PGF2α, During the reaction, 8-epi-PGF2α in the sample or standard competes with a fixed amount of biotin-labeled 8-epi-PGF2α for sites on a pre-coated Monoclonal antibody specific to 8-epi-PGF2α. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of 8-epi-PGF2α in the samples is then determined by comparing the O.D. of the samples to the standard curve.