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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSMBIO
- 检测范围:
0.15-10NG/ML
- 检测方法:
夹心法
- 应用:
夹心法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种:
人
- 标记物:
Human Spectrin beta chain,brain 1,SPTBN1
- 样本:
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
0.078NG/ML
- 规格:
96T
Human Spectrin beta chain, brain 1, SPTBN1 ELISA KIT
Product Name:Human Spectrin beta chain, brain 1, SPTBN1 ELISA KIT
Packing:96T
Catalog No.:ELI-53432h
Gene Name:SPTBN1
Detect Range:78-5000 pg/mL
Sensitivity:32pg/mL
Target Protein Name:Spectrin beta chain(brain 1(SPTBN1
Alternative Name:SPTBN1,Spectrin beta chain(brain 1(SPTBN1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Spectrin beta chain, brain 1, SPTBN1 ELISA KIT allows for the in vitro quantitative determination of Spectrin beta chain(brain 1(SPTBN1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Spectrin beta chain, brain 1, SPTBN1 ELISA KIT has been pre-coated with an Spectrin beta chain(brain 1(SPTBN1 antibody specific to Spectrin beta chain(brain 1(SPTBN1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Spectrin beta chain(brain 1(SPTBN1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Spectrin beta chain(brain 1(SPTBN1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Spectrin beta chain(brain 1(SPTBN1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Assay (Invitrogen) as suggested by Illumina. Illumina Bead Array MicroRNA Detection Starting with 500 ng/sample of total RNA, mature microRNAs were amplified with the Illumina human v1 MicroRNA expression profiling kit containing primers for 743
ELISPOT Assays Visualize the Secretory Product of Individual Cells
in the supernatant, captured by receptors of adjacent cells, or degraded. This makes ELISPOT assays much more sensitive than conventional ELISA measurements. Chronically stimulated T cells downregulate their TCR zeta-chain while maintaining unaltered levels
【资源】[共享]Adhesion Protein Protocols——(很有用的)
Notes References 2— Epitope Mapping 3— Sequencing of Antibodies 4— Purification through Affinity Chromatography and Microsequencing 5— Functional Analysis of Cell Adhesion Molecules 6— Application of Polymerase Chain Reaction
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