- ￥2890
- LSM Bio
- 0.15-10NG/ML
- 0.078NG/ML
- 进口
- ELI-52699h
- 2025年07月11日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSMBIO
- 检测范围：
0.15-10NG/ML
- 检测方法：
夹心法
- 应用：
夹心法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种：
人
- 标记物：
Human Ras-related GTP-binding protein C,RRAGC
- 样本：
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
0.078NG/ML
- 规格：
96T
Human Ras- related GTP- binding protein C, RRAGC ELISA KIT
Product Name:Human Ras- related GTP- binding protein C, RRAGC ELISA KIT
Packing:96T
Catalog No.:ELI-52699h
Gene Name:RRAGC
Detect Range:78-5000 pg/mL
Sensitivity:32pg/mL
Target Protein Name:Ras-related GTP-binding protein C(RRAGC
Alternative Name:RRAGC,Ras-related GTP-binding protein C(RRAGC
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Ras- related GTP- binding protein C, RRAGC ELISA KIT allows for the in vitro quantitative determination of Ras-related GTP-binding protein C(RRAGC concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Ras- related GTP- binding protein C, RRAGC ELISA KIT has been pre-coated with an Ras-related GTP-binding protein C(RRAGC antibody specific to Ras-related GTP-binding protein C(RRAGC .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Ras-related GTP-binding protein C(RRAGC and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Ras-related GTP-binding protein C(RRAGC, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Ras-related GTP-binding protein C(RRAGC in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Ras是大鼠肉瘤(rat sarcoma,Ras)的英文缩写。Ras蛋白是原癌基因 c—ras的表达产物，相对分子质量为21kDa，属单体 GTP结合蛋白，具有弱的 GTP酶活性。Ras蛋白的活性状态对细胞的生长、分化、细胞骨架、蛋白质运输和分泌等都具有影响，其活性则是通过与GTP或GDP的结合进行调节。 Ras的活性受两个蛋白的控制,一个是鸟苷交换因子(guanine nucleotide exchange factor, GEF),它的作用是促使GDP从Ras蛋白
ANTIBODY BINDING TO PROTEIN A AND PROTEIN G
Table I Species Protein A Protein G Human ++++ ++++ Mouse ++ ++ Rat
Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
between chromatin elements without specifically identifying the protein components that mediate such interactions. Here we describe a detailed protocol for C ombined 3C -C hIP-C loning (6C) technology, which combines multiple techniques to identify the proteins
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