Target Protein Name:Y+L amino acid transporter 1(SLC7A7
Alternative Name:SLC7A7,Y+L amino acid transporter 1(SLC7A7 Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit Product Description:Human Y+L amino acid transporter 1, SLC7A7 ELISA KIT allows for the in vitro quantitative determination of Y+L amino acid transporter 1(SLC7A7 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle: The microtiter plate provided in Human Y+L amino acid transporter 1, SLC7A7 ELISA KIT has been pre-coated with an Y+L amino acid transporter 1(SLC7A7 antibody specific to Y+L amino acid transporter 1(SLC7A7 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Y+L amino acid transporter 1(SLC7A7 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Y+L amino acid transporter 1(SLC7A7, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Y+L amino acid transporter 1(SLC7A7 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!