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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSMBIO
- 检测范围:
0.15-10NG/ML
- 检测方法:
夹心法
- 应用:
夹心法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种:
人
- 标记物:
Human Y+L amino acid transporter 1,SLC7A7
- 样本:
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
0.078NG/ML
- 规格:
96T
Human Y+L amino acid transporter 1, SLC7A7 ELISA KIT
Product Name:Human Y+L amino acid transporter 1, SLC7A7 ELISA KIT
Packing:96T
Catalog No.:ELI-51444h
Gene Name:SLC7A7
Detect Range:0.312-20 ng/mL
Sensitivity:0.039ng/mL
Target Protein Name:Y+L amino acid transporter 1(SLC7A7
Alternative Name:SLC7A7,Y+L amino acid transporter 1(SLC7A7
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Y+L amino acid transporter 1, SLC7A7 ELISA KIT allows for the in vitro quantitative determination of Y+L amino acid transporter 1(SLC7A7 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Y+L amino acid transporter 1, SLC7A7 ELISA KIT has been pre-coated with an Y+L amino acid transporter 1(SLC7A7 antibody specific to Y+L amino acid transporter 1(SLC7A7 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Y+L amino acid transporter 1(SLC7A7 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Y+L amino acid transporter 1(SLC7A7, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Y+L amino acid transporter 1(SLC7A7 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Studying Amino Acid Transport Using Liposomes
used technique to study amino acid transport was the measure of amino acid flux in intact cell systems expressing a specific transporter. Some limitations in this procedure are caused by the presence of endogenous transporters and intracellular enzymes
Isolated plasma membrane vesicles from human placenta allow transporter-mediated mechanisms across individual plasma membranes to be identified and characterized in vitro. This approach is reliant on isolating each of the trophoblast plasma
Antibody Production and Immunocytochemical Localization of Amino Acid Transporters
This chapter details the procedural and conceptual issues that underpin the localization of high-affinity amino acid transporters by immunocytochemistry, with particular reference to the mammalian nervous system. Defining a role
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