- ￥2890
- LSM Bio
- 0.31-20NG/ML
- 0.1NG/ML
- 进口
- ELI-51311h
- 2025年07月04日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSMBIO
- 检测范围：
0.31-20NG/ML
- 检测方法：
夹心法
- 应用：
夹心法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种：
人
- 标记物：
Human Ubiquitin carboxyl-terminal hydrolase 11,USP11
- 样本：
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
0.1NG/ML
- 规格：
96T
Human Ubiquitin carboxyl- terminal hydrolase 11, USP11 ELISA KIT
Product Name:Human Ubiquitin carboxyl- terminal hydrolase 11, USP11 ELISA KIT
Packing:96T
Catalog No.:ELI-51311h
Gene Name:USP11
Detect Range:0.156-10 ng/mL
Sensitivity:0.078ng/mL
Target Protein Name:Ubiquitin carboxyl-terminal hydrolase 11(USP11
Alternative Name:USP11,Ubiquitin carboxyl-terminal hydrolase 11(USP11
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Ubiquitin carboxyl- terminal hydrolase 11, USP11 ELISA KIT allows for the in vitro quantitative determination of Ubiquitin carboxyl-terminal hydrolase 11(USP11 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Ubiquitin carboxyl- terminal hydrolase 11, USP11 ELISA KIT has been pre-coated with an Ubiquitin carboxyl-terminal hydrolase 11(USP11 antibody specific to Ubiquitin carboxyl-terminal hydrolase 11(USP11 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Ubiquitin carboxyl-terminal hydrolase 11(USP11 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Ubiquitin carboxyl-terminal hydrolase 11(USP11, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Ubiquitin carboxyl-terminal hydrolase 11(USP11 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Studies of the Ubiquitin Proteasome System
Protocol 10: Detection of E3 Activity in Immunoprecipitated Protein Alternate Protocol 5: Detection of Ubiquitin Modification of Substrates After GST Pull‐Down Detection of Ubiquitylation in Vivo Basic Protocol 11: Lysis and Immunoprecipitation
【分享】Human press METHODS IN MOLECULAR BIOLOGY
9. Protocols in Human Molecular Genetics. Edited by G. Methaw, 1991 10. Immunochemical Protocols. Edited by Manson, Margaret M., 1992 11. Practical Protein Chromatography Edited by Kenney, Andrew, 1992 12. Pulsed-Field Gel Electrophoresis Protocols
（Ubiquitin C-terminal hydrolase-L1，UCH-L1）是一种主要分布于脑内，同时对人类大脑具有高度特异性的球蛋白。 癫痫大发作组脑脊液 UCH-L1 水平显著高于部分性发作组和对照组。癫痫患者的脑脊液 UCHL1 水平与癫痫发作的严重程度和癫痫发作的持续时间密切相关。癫痫发作后 6h 内检验患者血清 UCH-L1 及 S100β 水平，结果显示癫痫组 UCHL-1 及 S100β 水平显著高于精神源性非癫痫性发作组及健康人群组，精神源性非癫痫性发作组 S100β 水平显著高于健康对照
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