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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSMBIO
- 检测范围:
0.31-20NG/ML
- 检测方法:
夹心法
- 应用:
夹心法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种:
人
- 标记物:
Human Zinc finger protein 280C,ZNF280C
- 样本:
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
0.1NG/ML
- 规格:
96T
Human Zinc finger protein 280C, ZNF280C ELISA KIT
Product Name:Human Zinc finger protein 280C, ZNF280C ELISA KIT
Packing:96T
Catalog No.:ELI-51169h
Gene Name:ZNF280C
Detect Range:0.312-20 ng/mL
Sensitivity:0.039ng/mL
Target Protein Name:Zinc finger protein 280C(ZNF280C
Alternative Name:ZNF280C,Zinc finger protein 280C(ZNF280C
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Zinc finger protein 280C, ZNF280C ELISA KIT allows for the in vitro quantitative determination of Zinc finger protein 280C(ZNF280C concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Zinc finger protein 280C, ZNF280C ELISA KIT has been pre-coated with an Zinc finger protein 280C(ZNF280C antibody specific to Zinc finger protein 280C(ZNF280C .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Zinc finger protein 280C(ZNF280C and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Zinc finger protein 280C(ZNF280C, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Zinc finger protein 280C(ZNF280C in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Gene Editing in One‐Cell Embryos by Zinc‐Finger and TAL Nucleases
., and Cui, X. 2010. Targeted genome modification in mice using zinc‐finger nucleases. Genetics 186:451‐459. Cermak, T., Doyle, E.L., Christian, M., Wang, L., Zhang, Y., Schmidt, C
were then cloned into a yeast expression plasmid that contained the Gal4-Zif268 hybrid transcription factor. In this library, zinc finger 3 (the C-terminal finger) of Zif268 was replaced with zinc-finger sequences amplified from the human genome (Fig. 1A).
【分享】Human press METHODS IN MOLECULAR BIOLOGY
9. Protocols in Human Molecular Genetics. Edited by G. Methaw, 1991 10. Immunochemical Protocols. Edited by Manson, Margaret M., 1992 11. Practical Protein Chromatography Edited by Kenney, Andrew, 1992 12. Pulsed-Field Gel Electrophoresis Protocols
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