- ￥2890
- LSM Bio
- 0.15-10NG/ML
- 0.078NG/ML
- 进口
- ELI-51055h
- 2025年07月11日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSMBIO
- 检测范围：
0.15-10NG/ML
- 检测方法：
夹心法
- 应用：
夹心法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种：
人
- 标记物：
Human TNF receptor-associated factor 4,TRAF4
- 样本：
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
0.078NG/ML
- 规格：
96T
Human TNF receptor- associated factor 4, TRAF4 ELISA KIT
Product Name:Human TNF receptor- associated factor 4, TRAF4 ELISA KIT
Packing:96T
Catalog No.:ELI-51055h
Gene Name:TRAF4
Detect Range:78-5000 pg/mL
Sensitivity:32pg/mL
Target Protein Name:TNF receptor-associated factor 4(TRAF4
Alternative Name:TRAF4,TNF receptor-associated factor 4(TRAF4
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human TNF receptor- associated factor 4, TRAF4 ELISA KIT allows for the in vitro quantitative determination of TNF receptor-associated factor 4(TRAF4 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human TNF receptor- associated factor 4, TRAF4 ELISA KIT has been pre-coated with an TNF receptor-associated factor 4(TRAF4 antibody specific to TNF receptor-associated factor 4(TRAF4 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for TNF receptor-associated factor 4(TRAF4 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain TNF receptor-associated factor 4(TRAF4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of TNF receptor-associated factor 4(TRAF4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Detection of TNF and TNF Receptor mRNA in Cells and Tissues
We describe a semiquantitative reverse transcriptase polymerase chain reaction (RT-PCR) technique for the quantification of the messenger RNA of human and murine tumor necrosis factor α (TNFα) and related receptors. This protocol can be adapted
Production and Characterization of Receptor-Specific TNF Muteins
Tumor necrosis factor (TNF) is a pleiotropic cytokine with a wide range of biological activities including cytotoxicity, immune-cell proliferation, and mediation of inflammatory responses. Mutational analysis of mature TNF has been facilitated
Production and Characterization of Recombinant Human and Murine TNF
and the identity of the final products can be checked by SDS-PAGE, ELISA, Western blot, analytical gel-filtration chromatography, mass spectrometry, and lipopolysaccharide assay. The biological activity of both human and murine TNF is assessed by in vitro cytolytic
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