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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSMBIO
- 检测范围:
0.15-10NG/ML
- 检测方法:
夹心法
- 应用:
夹心法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种:
人
- 标记物:
Human Bone marrow stromal antigen 2,BST2
- 样本:
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
0.078NG/ML
- 规格:
96T
Human Bone marrow stromal antigen 2, BST2 ELISA KIT
Product Name:Human Bone marrow stromal antigen 2, BST2 ELISA KIT
Packing:96T
Catalog No.:ELI-50429h
Gene Name:BST2
Detect Range:78-5000 pg/mL
Sensitivity:32pg/mL
Target Protein Name:Bone marrow stromal antigen 2(BST2
Alternative Name:BST2,Bone marrow stromal antigen 2(BST2
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Bone marrow stromal antigen 2, BST2 ELISA KIT allows for the in vitro quantitative determination of Bone marrow stromal antigen 2(BST2 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Bone marrow stromal antigen 2, BST2 ELISA KIT has been pre-coated with an Bone marrow stromal antigen 2(BST2 antibody specific to Bone marrow stromal antigen 2(BST2 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Bone marrow stromal antigen 2(BST2 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Bone marrow stromal antigen 2(BST2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Bone marrow stromal antigen 2(BST2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验A Method to Isolate and Purify Human Bone Marrow Stromal Stem Cells
The STRO-1 antibody can be used as a single reagent to isolate human bone marrow stromal stem cells (BMSSC), owing to its restricted specificity to a cell surface molecule expressed by clonogenic BMSSC, with little or no reactivity
Isolation and Culture of Bone Marrow-Derived Human Multipotent Stromal Cells (hMSCs)
to generate as many as 5 � 108 multipotent stromal cells (MSCs) from a single sample, depending on the number of Cell Factories seeded from the initial isolated hMSCs. Briefly, mononuclear cells are collected from a bone marrow aspirate by density gradient
In recent years, human mesenchymal stem cells (multipotential stromal cells) from bone marrow (hMSCs) have attracted enormous attention owing to their broad therapeutic potential. One of the problems in the overall therapeutic use of hMSCs
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