Human CDC42 small effector protein 2, CDC42SE2 ELISA KIT
Product Name:Human CDC42 small effector protein 2, CDC42SE2 ELISA KIT Packing:96T
Catalog No.:ELI-50152h
Gene Name:CDC42SE2
Detect Range:0.312-20 ng/mL
Sensitivity:0.039ng/mL
Target Protein Name:CDC42 small effector protein 2(CDC42SE2
Alternative Name:CDC42SE2,CDC42 small effector protein 2(CDC42SE2 Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit Product Description:Human CDC42 small effector protein 2, CDC42SE2 ELISA KIT allows for the in vitro quantitative determination of CDC42 small effector protein 2(CDC42SE2 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle: The microtiter plate provided in Human CDC42 small effector protein 2, CDC42SE2 ELISA KIT has been pre-coated with an CDC42 small effector protein 2(CDC42SE2 antibody specific to CDC42 small effector protein 2(CDC42SE2 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for CDC42 small effector protein 2(CDC42SE2 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain CDC42 small effector protein 2(CDC42SE2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of CDC42 small effector protein 2(CDC42SE2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!