- ￥2890
- LSM Bio
- 0.15-10NG/ML
- 0.078NG/ML
- 进口
- ELI-49778h
- 2025年07月10日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSMBIO
- 检测范围：
0.15-10NG/ML
- 检测方法：
夹心法
- 应用：
夹心法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种：
人
- 标记物：
Human HLA class I histocompatibility antigen,Cw-18 alpha chain
- 样本：
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
0.078NG/ML
- 规格：
96T
Human HLA class I histocompatibility antigen,Cw-18 alpha chainHLA-C ELISA KIT
Product Name:Human HLA class I histocompatibility antigen,Cw-18 alpha chainHLA-C ELISA KIT
Packing:96T
Catalog No.:Human HLA class I histocompatibility antigen,Cw-18 alpha chainHLA-C ELISA KIT
Gene Name:Human HLA class I histocompatibility antigen,Cw-18 alpha chainHLA-C
Detect Range:0.312-20 ng/mL
Sensitivity:0.039ng/mL
Target Protein Name:Human HLA class I histocompatibility antigen,Cw-18 alpha chainHLA-C
Alternative Name:Human HLA class I histocompatibility antigen,Cw-18 alpha chainHLA-C
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human HLA class I histocompatibility antigen,Cw-18 alpha chainHLA-C ELISA KIT allows for the in vitro quantitative determination of Human HLA class I histocompatibility antigen,Cw-18 alpha chainHLA-C ELISA KIT concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human HLA class I histocompatibility antigen,Cw-18 alpha chainHLA-C ELISA KIT has been pre-coated with an Human HLA class I histocompatibility antigen,Cw-18 alpha chainHLA-C antibody specific to Human HLA class I histocompatibility antigen,Cw-18 alpha chainHLA-C .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Human HLA class I histocompatibility antigen,Cw-18 alpha chainHLA-C and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human HLA class I histocompatibility antigen,Cw-18 alpha chainHLA-C, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Human HLA class I histocompatibility antigen,Cw-18 alpha chainHLA-C in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Major histocompatibility complex (MHC) class I human genes have been divided into two different subgroups. The classical class I loci, human leukocyte antigen (HLA)-A, -B and -C, encode highly polymorphic membrane glycoproteins
人白细胞抗原(human leukocyte antigen，HLA)
gene)。人的定位MHC在第6号染色体上，小鼠的则定位在第17号染色体上。 组织相容性抗原按其免疫性能可分为三类：第一类是移植抗原(transplanta—tion antigen)，负责排斥外来的组织，存在于杀伤性T淋巴细胞(killer T leukocyte)中，为细胞介导的免疫反应所必需，小鼠的I类基因编码H2-K和H2-D／L蛋白质，人的I类基因编码移植抗原HLA-A，B，C；第二类是参与免疫反应过程中细胞之间的信息传递，人的Ⅱ类区称为HLA-D，又可分为4个亚区，排列顺序为DR，DQ
会（International Histocompatibility Workshop,IHW）,最近一次于1991年11月在日本横滨召开，并预定于1995年在法国召开第12次IHW。经过这些 会议 陆续报告了HLA的许多基因及大量的行装位基因。现知在HLA-I类基因区中，除已知的HLA-A、-B、-C座位外，还发现了-E、-F、-G、-H和-J，新发现的这些I因基因座大多数伪基因。现A座位已发现等位基因41个，B座位61个，C座位18个，E座位4个。在HLA-A与-E之间可能存在着重组热点。在HLA
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