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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSMBIO
- 检测范围:
0.31-20NG/ML
- 检测方法:
夹心法
- 应用:
夹心法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种:
人
- 标记物:
Human HLA class I histocompatibility antigen,Cw-17 alpha chain
- 样本:
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
0.1NG/ML
- 规格:
96T
Human HLA class I histocompatibility antigen,Cw-17 alpha chainHLA-C ELISA KIT
Product Name:Human HLA class I histocompatibility antigen,Cw-17 alpha chainHLA-C ELISA KIT
Packing:96T
Catalog No.:Human HLA class I histocompatibility antigen,Cw-17 alpha chainHLA-C ELISA KIT
Gene Name:Human HLA class I histocompatibility antigen,Cw-17 alpha chainHLA-C
Detect Range:0.312-20 ng/mL
Sensitivity:0.039ng/mL
Target Protein Name:Human HLA class I histocompatibility antigen,Cw-17 alpha chainHLA-C
Alternative Name:Human HLA class I histocompatibility antigen,Cw-17 alpha chainHLA-C
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human HLA class I histocompatibility antigen,Cw-17 alpha chainHLA-C ELISA KIT allows for the in vitro quantitative determination of Human HLA class I histocompatibility antigen,Cw-17 alpha chainHLA-C ELISA KIT concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human HLA class I histocompatibility antigen,Cw-17 alpha chainHLA-C ELISA KIT has been pre-coated with an Human HLA class I histocompatibility antigen,Cw-17 alpha chainHLA-C antibody specific to Human HLA class I histocompatibility antigen,Cw-17 alpha chainHLA-C .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Human HLA class I histocompatibility antigen,Cw-17 alpha chainHLA-C and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human HLA class I histocompatibility antigen,Cw-17 alpha chainHLA-C, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Human HLA class I histocompatibility antigen,Cw-17 alpha chainHLA-C in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验人白细胞抗原(human leukocyte antigen,HLA)
gene)。人的定位MHC在第6号染色体上,小鼠的则定位在第17号染色体上。 组织相容性抗原按其免疫性能可分为三类:第一类是移植抗原(transplanta—tion antigen),负责排斥外来的组织,存在于杀伤性T淋巴细胞(killer T leukocyte)中,为细胞介导的免疫反应所必需,小鼠的I类基因编码H2-K和H2-D/L蛋白质,人的I类基因编码移植抗原HLA-A,B,C;第二类是参与免疫反应过程中细胞之间的信息传递,人的Ⅱ类区称为HLA-D,又可分为4个亚区,排列顺序为DR,DQ
Major histocompatibility complex (MHC) class I human genes have been divided into two different subgroups. The classical class I loci, human leukocyte antigen (HLA)-A, -B and -C, encode highly polymorphic membrane glycoproteins
网络 二、人HLA基因图及其遗传特征 在人类MHC称为HLL(human leucocyte antigen),是迄今为止所知的人类最复杂的基因族。除成熟的红细胞外,HLA抗原几科分布于人体的各种有核细胞以及血小板。由于此组抗原首先在人外周血白细胞上发现,同时表达抗原水平较高,目前多采用外周血淋巴细胞来检测这类抗原的型别,故称为人类白细胞抗原(HLA
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