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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSMBIO
- 检测范围:
0.31-20NG/ML
- 检测方法:
夹心法
- 应用:
夹心法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种:
人
- 标记物:
Human ADAM DEC1,ADAMDEC1
- 样本:
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
0.1NG/ML
- 规格:
96T
Human ADAM DEC1, ADAMDEC1 ELISA KIT
Product Name:Human ADAM DEC1, ADAMDEC1 ELISA KIT
Packing:96T
Catalog No.:ELI-49696h
Gene Name:ADAMDEC1
Detect Range:0.156-10 ng/mL
Sensitivity:0.078ng/mL
Target Protein Name:ADAM DEC1(ADAMDEC1
Alternative Name:ADAMDEC1,ADAM DEC1(ADAMDEC1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human ADAM DEC1, ADAMDEC1 ELISA KIT allows for the in vitro quantitative determination of ADAM DEC1(ADAMDEC1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human ADAM DEC1, ADAMDEC1 ELISA KIT has been pre-coated with an ADAM DEC1(ADAMDEC1 antibody specific to ADAM DEC1(ADAMDEC1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for ADAM DEC1(ADAMDEC1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain ADAM DEC1(ADAMDEC1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of ADAM DEC1(ADAMDEC1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验有替代前者的趋势。由于ABS-ELISA较普通ELISA多用了两种试剂,增加了操作步骤,在临床检验中ABS-ELISA应用不多。科研项目中检测微量的成分如细胞因子常采用本法。晶美分装ELISA KIT采用的方法:1, TORCH及传染病试剂盒(间接法),见2.2.32, TORCH-IgM捕获法特色:包被抗体,标记抗原原理:3, 细胞因子试剂盒采用的方法路线(ABC-ELISA)原理产品特色:采用ABC法,灵敏度更高,特异性更强。生物素抗体和酶联物是浓缩的,使用前需用相应的缓冲液稀释。酶联物可以通用
的原理用于分子诊断方面:TrimGen公司的KRAS Mutation Detection Kit在96孔板里一次能实现7/8个KRAS突变位点的检测。 另外基于相同原理的技术还有PPT-ELISA。这是用来筛截短突变的,相关文章见:1,Rapid screen for truncating ATM mutations by PTT-ELISA . 2,An ELISA-based high throughput protein truncation test
相关专题 NCBI-做到最全最强大 可借此问题介绍3个主要的基因组 浏览器。将利用所有3个站点对基因ADAM2进行检测,使读者能对每个站点提供的信息之间的细微的区别有一个正确的认识。 1.国立生物技术信息中心(NCBI)图谱 浏览器(Map Viewer) 可以通过NCBI主页进入NCBI的人类图谱 浏览器,网址为http://www.ncbi.nlm.nih.gov/。点击右栏标有“Human map viewer
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