This immunoassay kit allows for the in vitro quantitative determination of Human DTW domain-containing protein 2concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.
The ELISA is based on the competitive binding enzyme immunoassaytechnique. The microtiter plate provided in this kit has been pre-coated with an antibody specific toDTW domain-containing protein 2, During the reaction,DTW domain-containing protein 2in the sample or standard competes with a fixed amount of biotin-labeledDTW domain-containing protein 2 for sites on a pre-coated Monoclonal antibody specific to DTW domain-containing protein 2. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Thena TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of DTW domain-containing protein 2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.