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- 详细信息
- 文献和实验
- 技术资料
- 库存:
20
- 供应商:
LSMBIO
- 检测范围:
0.31-20ng/Ml
- 检测方法:
夹心法
- 应用:
夹心法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种:
人
- 标记物:
Human DDB1- and CUL4-associated factor 7,DCAF7
- 样本:
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
0.1ng/Ml
- 规格:
96T
Human DDB1- and CUL4- associated factor 7, DCAF7 ELISA KIT
Product Name:Human DDB1- and CUL4- associated factor 7, DCAF7 ELISA KIT
Packing:96T
Catalog No.:ELI-47000h
Gene Name:DCAF7
Detect Range:0.312-20 ng/mL
Sensitivity:0.039ng/mL
Target Protein Name:DDB1- and CUL4-associated factor 7(DCAF7
Alternative Name:DCAF7,DDB1- and CUL4-associated factor 7(DCAF7
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human DDB1- and CUL4- associated factor 7, DCAF7 ELISA KIT allows for the in vitro quantitative determination of DDB1- and CUL4-associated factor 7(DCAF7 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human DDB1- and CUL4- associated factor 7, DCAF7 ELISA KIT has been pre-coated with an DDB1- and CUL4-associated factor 7(DCAF7 antibody specific to DDB1- and CUL4-associated factor 7(DCAF7 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for DDB1- and CUL4-associated factor 7(DCAF7 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain DDB1- and CUL4-associated factor 7(DCAF7, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of DDB1- and CUL4-associated factor 7(DCAF7 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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时对一个样本中的75个目的蛋白进行精确定量。2、样本需要量小。仅需要50μL溶液就可进行一次检测。3、更高的灵敏度。分析灵敏度高达2.8pg/mL。4、更高的灵敏度、更宽的检测范围和更好的重复性。CBA技术使蛋白定量的分析灵敏度高达pg/ml级别,检测范围达0-5000pg/ml,所有分析只需一组标准曲线。根据抗体的荧光强度对目的蛋白定量,排除了ELISA反应中酶联放大产生的假阳性。5、检测对象的灵活组合—BD CBA Flex Set。CBA kit是同时检测试剂盒配好的6个指标,形式比较固定
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