- ￥2890
- LSM Bio
- 0.31-20ng/Ml
- 0.1ng/Ml
- 进口
- ELI-46087h
- 2025年07月16日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
20
- 供应商：
LSMBIO
- 检测范围：
0.31-20ng/Ml
- 检测方法：
夹心法
- 应用：
夹心法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种：
人
- 标记物：
Human Nuclear pore complex protein Nup133,NUP133
- 样本：
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
0.1ng/Ml
- 规格：
96T
Human Nuclear pore complex protein Nup133, NUP133 ELISA KIT
Product Name:Human Nuclear pore complex protein Nup133, NUP133 ELISA KIT
Packing:96T
Catalog No.:ELI-46087h
Gene Name:NUP133
Detect Range:0.312-20 ng/mL
Sensitivity:0.039ng/mL
Target Protein Name:Nuclear pore complex protein Nup133(NUP133
Alternative Name:NUP133,Nuclear pore complex protein Nup133(NUP133
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Nuclear pore complex protein Nup133, NUP133 ELISA KIT allows for the in vitro quantitative determination of Nuclear pore complex protein Nup133(NUP133 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Nuclear pore complex protein Nup133, NUP133 ELISA KIT has been pre-coated with an Nuclear pore complex protein Nup133(NUP133 antibody specific to Nuclear pore complex protein Nup133(NUP133 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Nuclear pore complex protein Nup133(NUP133 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Nuclear pore complex protein Nup133(NUP133, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Nuclear pore complex protein Nup133(NUP133 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验施一公团队突破性进展再登 Science！以超高分辨率解析核孔复合物的结构细节
质组装成正确的三维分子结构一直是一个巨大的挑战。 2022 年 6 月 10 日，西湖大学施一公团队在 Science 杂志发表研究论文 Structure of the cytoplasmic ring of the Xenopus laevis nuclear pore complex。 该研究聚焦于非洲爪蟾核孔复合物（NPC）结构，首次扩展了脊椎动物细胞质环（CR）亚单位复合体模型的分子量，获得高分辨率的单粒子冷冻电镜显微结构。除了解析 Y 复合物，同时发现 5 个核孔蛋白 Nup358
Noninvasive Human Nuclear Transfer with Embryonic Stem Cells
Noninvasive Human Nuclear Transfer with Embryonic Stem Cells Sohyun L. McElroy1 and Renee A. Reijo Pera Center for Human Embryonic Stem Cell Research and Education, Institute for Stem Cell Biology and Regenerative Medicine
at 4°C. Transfer supernatant (nuclear fraction) to a clean microcentrifuge tube. 11. Aliquot and store at –80°C. The nuclear extracts are ready for assay. 12. Quantitate protein concentration using the Quant-iT™ Protein Quantitation Kit (Cat. no. Q
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