- ￥2890
- LSM Bio
- 0.156-10ng/ml
- 0.078ng/ml
- 进口
- ELI-43234h
- 2025年07月14日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSMBIO
- 检测范围：
0.156-10ng/ml
- 检测方法：
夹心法
- 应用：
夹心法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种：
人
- 标记物：
Human Poly(ADP-ribose) glycohydrolase,PARG
- 样本：
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
0.078ng/ml
- 规格：
96T
Human Poly(ADP- ribose) glycohydrolase, PARG ELISA KIT
Product Name:Human Poly(ADP- ribose) glycohydrolase, PARG ELISA KIT
Packing:96T
Catalog No.:ELI-43234h
Gene Name:PARG
Detect Range:0.156-10 ng/mL
Sensitivity:0.078ng/mL
Target Protein Name:Poly(ADP-ribose) glycohydrolase(PARG
Alternative Name:PARG,Poly(ADP-ribose) glycohydrolase(PARG
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Poly(ADP- ribose) glycohydrolase, PARG ELISA KIT allows for the in vitro quantitative determination of Poly(ADP-ribose) glycohydrolase(PARG concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Poly(ADP- ribose) glycohydrolase, PARG ELISA KIT has been pre-coated with an Poly(ADP-ribose) glycohydrolase(PARG antibody specific to Poly(ADP-ribose) glycohydrolase(PARG .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Poly(ADP-ribose) glycohydrolase(PARG and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Poly(ADP-ribose) glycohydrolase(PARG, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Poly(ADP-ribose) glycohydrolase(PARG in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Poly(ADP-Ribose) Metabolism Analysis in the Nematode Caenorhabditis elegans
signaling and repair, cell death and survival, and transcription. Poly(ADP-ribose) glycohydrolase (PARG) is responsible for the specific hydrolysis of poly(ADP-ribose) (PAR), the product of poly(ADP-ribosyl)ation, and its action is required for the modified
poly(ADP-ribose) polymerase-1. We have been studying to analyze the mechanism of neuronal cell death observed in poly(ADP-ribose) glycohydrolase (PARG)-knockout Drosophila melanogaster that shows accumulation of polyADP-ribosylated proteins in the brain
) is degraded by the endo- and exo-glycosidase activities of poly(ADP-ribose) glycohydrolase (PARG). In this chapter, biochemical and immunofluorescence methods are described for detecting and assaying PARPs and PARG.
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