Human Calcium /calmodulin-depe

Human Calcium/calmodulin- dependent protein kinase type 1D, CAMK1D ELISA KIT

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

Camk1d,CKLiK,CaM-K1; CaMKID; CaM kinase ID; CamKI-like protein kinase; caM kinase I delta; caM-KI delta; caMKI delta; calcium/calmodulin-dependent protein kinase type 1D; calcium/calmodulin-dependent protein kinase ID

Search name

Human Camk1d ELISA KIT ,Human CKLiK ELISA KIT ,Human CaM-K1 ELISA KIT ,Human CaMKID ELISA KIT ,Human CaM kinase ID ELISA KIT ,Human CamKI-like protein kinase ELISA KIT ,Human caM kinase I delta ELISA KIT ,Human caM-KI delta ELISA KIT ,Human caMKI delta ELISA KIT ,Human calcium/calmodulin-dependent protein kinase type 1D ELISA KIT ,Human calcium/calmodulin-dependent protein kinase ID ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Human Calcium/calmodulin- dependent protein kinase type 1D, CAMK1D concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to CAMK1D. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for CAMK1D and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain CAMK1D, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of CAMK1D in the samples is then determined by comparing the O.D. of the samples to the standard curve.