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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSMBIO
- 检测范围:
0.156-10ng/ml
- 检测方法:
夹心法
- 应用:
夹心法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种:
人
- 标记物:
Human Casein kinase I isoform alpha,CSNK1A1
- 样本:
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
0.078ng/ml
- 规格:
96T
Human Casein kinase I isoform alpha, CSNK1A1 ELISA KIT
Product Name:Human Casein kinase I isoform alpha, CSNK1A1 ELISA KIT
Packing:96T
Catalog No.:ELI-42975h
Gene Name:CSNK1A1
Detect Range:0.156-10 ng/mL
Sensitivity:0.078ng/mL
Target Protein Name:Casein kinase I isoform alpha(CSNK1A1
Alternative Name:CSNK1A1,Casein kinase I isoform alpha(CSNK1A1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Casein kinase I isoform alpha, CSNK1A1 ELISA KIT allows for the in vitro quantitative determination of Casein kinase I isoform alpha(CSNK1A1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Casein kinase I isoform alpha, CSNK1A1 ELISA KIT has been pre-coated with an Casein kinase I isoform alpha(CSNK1A1 antibody specific to Casein kinase I isoform alpha(CSNK1A1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Casein kinase I isoform alpha(CSNK1A1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Casein kinase I isoform alpha(CSNK1A1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Casein kinase I isoform alpha(CSNK1A1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Introduction of human 14-3-3 proteins
regulation.We report here the crystal structure of the human T-cell 14-3-3 isoform (tau)dimer at 2.6 A resolution.Each monomer (Mr 28K)is composed of an unusual arrangement of nine antiparallel alpha-helices organized as two structural domains.The dimer
Assay (Invitrogen) as suggested by Illumina. Illumina Bead Array MicroRNA Detection Starting with 500 ng/sample of total RNA, mature microRNAs were amplified with the Illumina human v1 MicroRNA expression profiling kit containing primers for 743
Using Phospho‐Motif Antibodies to Determine Kinase Substrates
Manning, G., Whyte, D.B., Martinez, R., Hunter, T., and Sudarsanam, S. 2002b. The protein kinase complement of the human genome. Science 298:1912‐1934.
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