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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSMBIO
- 检测范围:
0.312-20ng/ml
- 检测方法:
夹心法
- 应用:
夹心法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种:
人
- 标记物:
Human Regulator of G-protein signaling 16,RGS16
- 样本:
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
0.1ng/ml
- 规格:
96T
Human Regulator of G- protein signaling 16, RGS16 ELISA KIT
Product Name:Human Regulator of G- protein signaling 16, RGS16 ELISA KIT
Packing:96T
Catalog No.:ELI-42742h
Gene Name:RGS16
Detect Range:78-5000 pg/mL
Sensitivity:32pg/mL
Target Protein Name:Regulator of G-protein signaling 16(RGS16
Alternative Name:RGS16,Regulator of G-protein signaling 16(RGS16
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Regulator of G- protein signaling 16, RGS16 ELISA KIT allows for the in vitro quantitative determination of Regulator of G-protein signaling 16(RGS16 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Regulator of G- protein signaling 16, RGS16 ELISA KIT has been pre-coated with an Regulator of G-protein signaling 16(RGS16 antibody specific to Regulator of G-protein signaling 16(RGS16 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Regulator of G-protein signaling 16(RGS16 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Regulator of G-protein signaling 16(RGS16, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Regulator of G-protein signaling 16(RGS16 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Evaluating Modulators of “Regulator of G‐protein Signaling” (RGS) Proteins
), and for validation of candidate GAP?modulatory molecules with the single?turnover GTP hydrolysis assay. Curr. Protoc. Pharmacol. 56:2.8.1?2.8.15. © 2012 by John Wiley & Sons, Inc. Keywords: regulator of G?protein signaling (RGS) proteins
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