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- 文献和实验
- 技术资料
- 库存:
20
- 供应商:
LSMBIO
- 检测范围:
0.156-10ng/ml
- 检测方法:
夹心法
- 应用:
夹心法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种:
人
- 标记物:
Human Protein Hook homolog 3,HOOK3
- 样本:
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
0.078ng/ml
- 规格:
96T
Human Protein Hook homolog 3, HOOK3 ELISA KIT
Product Name:Human Protein Hook homolog 3, HOOK3 ELISA KIT
Packing:96T
Catalog No.:ELI-42711h
Gene Name:HOOK3
Detect Range:0.312-20 ng/mL
Sensitivity:0.039ng/mL
Target Protein Name:Protein Hook homolog 3(HOOK3
Alternative Name:HOOK3,Protein Hook homolog 3(HOOK3
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Protein Hook homolog 3, HOOK3 ELISA KIT allows for the in vitro quantitative determination of Protein Hook homolog 3(HOOK3 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Protein Hook homolog 3, HOOK3 ELISA KIT has been pre-coated with an Protein Hook homolog 3(HOOK3 antibody specific to Protein Hook homolog 3(HOOK3 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Protein Hook homolog 3(HOOK3 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Protein Hook homolog 3(HOOK3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Protein Hook homolog 3(HOOK3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验ELISA信号太弱/不显色全白板怎么办?学会这几招拯救你的实验!
,则大概率是Hook效应。 4、排查样品自身的问题:反复冻融导致目标蛋白降解或聚集;未加蛋白酶抑制剂,目标物被降解;离心不充分,颗粒物干扰;前处理回收率低(提取、浓缩、超滤步骤的丢失);稀释倍数过大等。 斯达特(STARTER)One-step ELISA优质之选 Starter基于单B细胞的兔单克隆抗体开发平台(S-RMab® Platform),使用高灵敏抗体和创新性技术,开发全新一步法ELISA试剂盒, 1小时即可完成实验。 部分热卖产品 货号 名称 S0C3005 Human
Synchronizing Protein Transport in the Secretory Pathway
Figure 15.19.1 Principle of the RUSH system. The RUSH (retention using selective hooks) system is a two‐state secretory assay. The reporter protein is retained in the donor compartment via interaction with the hook mediated by the streptavidin‐SBP
【资源】[共享]Adhesion Protein Protocols——(很有用的)
Contents: Table of Contents Adhesion Protein Protocols Preface Contents Contributors 1— Monoclonal Antibodies Specific for Leukocyte Adhesion Molecules Selective Protocols of Immunizati... 1— Introduction 2— Materials 3— Methods 4—
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