- ￥2890
- LSM Bio
- 0.156-10ng/ml
- 0.078ng/ml
- 国产(香港)
- ELI-42445h
- 2026年05月21日
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- 文献和实验
- 技术资料
- 库存：
20
- 供应商：
LSMBIO
- 检测范围：
0.156-10ng/ml
- 检测方法：
夹心法
- 应用：
夹心法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种：
人
- 标记物：
Human Sorting nexin-6,SNX6
- 样本：
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
0.078ng/ml
- 规格：
96T
Human Sorting nexin- 6, SNX6 ELISA KIT
Product Name:Human Sorting nexin- 6, SNX6 ELISA KIT
Packing:96T
Catalog No.:ELI-42445h
Gene Name:SNX6
Detect Range:0.156-10 ng/mL
Sensitivity:0.078ng/mL
Target Protein Name:Sorting nexin-6(SNX6
Alternative Name:SNX6,Sorting nexin-6(SNX6
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Sorting nexin- 6, SNX6 ELISA KIT allows for the in vitro quantitative determination of Sorting nexin-6(SNX6 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Sorting nexin- 6, SNX6 ELISA KIT has been pre-coated with an Sorting nexin-6(SNX6 antibody specific to Sorting nexin-6(SNX6 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Sorting nexin-6(SNX6 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Sorting nexin-6(SNX6, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Sorting nexin-6(SNX6 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验the beadbound cells with a magnet. Discard the bead-bound cells and use the remaining untouched, enriched cell population for further flow sorting into the DC subpopulation(s) of interest 实验试剂 Magnet: (Dynal® MPC™), MPC-L for 1–5 ml
调至 1μg/μl。 4. ELISA 检测：按试剂盒说明书完成板平衡、加样孵育、洗板、酶标抗体孵育、显色终止与读数，结合标准曲线得出 IFN-γ 浓度。 四、操作要点速记 1.组织样本新鲜处理，全程低温操作，裂解液现配现用； 2. 蛋白定量时标准品与样本同步孵育，稀释采用倍比稀释减少误差； 3. ELISA 实验需每次重绘标准曲线，洗板充分、严格把控显色与终止时间。 免疫检测试剂盒： 货号-规格 名称 abs510001-96T Human IL-2 ELISA Kit (Faster
操作条件、人员一致 边缘效应 蒸发/酶标板叠放 孵育加封板膜或盖板；避免叠放 孵育温度不均 校准温育箱；避开通风口、窗边等环境波动区 洗板不彻底 确保洗板仪正常；不减少洗液量与次数 注意！！！ ELISA试剂盒11月活动即将来袭~敬请期待~ 部分热卖产品推荐 产品货号 产品名称 检测范围 灵敏度 97109ES Human IL18 ELISA Kit 125
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