- ￥2890
- LSM Bio
- 0.312-20ng/ml
- 0.1ng/ml
- 进口
- ELI-41163h
- 2025年07月13日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSMBIO
- 检测范围：
0.312-20ng/ml
- 检测方法：
夹心法
- 应用：
夹心法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种：
人
- 标记物：
Human E3 ubiquitin ligase RNF4,RNF4
- 样本：
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
0.1ng/ml
- 规格：
96T
Human E3 ubiquitin ligase RNF4, RNF4 ELISA KIT
Product Name:Human E3 ubiquitin ligase RNF4, RNF4 ELISA KIT
Packing:96T
Catalog No.:ELI-41163h
Gene Name:RNF4
Detect Range:0.156-10 ng/mL
Sensitivity:0.078ng/mL
Target Protein Name:E3 ubiquitin ligase RNF4(RNF4
Alternative Name:RNF4,E3 ubiquitin ligase RNF4(RNF4
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human E3 ubiquitin ligase RNF4, RNF4 ELISA KIT allows for the in vitro quantitative determination of E3 ubiquitin ligase RNF4(RNF4 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human E3 ubiquitin ligase RNF4, RNF4 ELISA KIT has been pre-coated with an E3 ubiquitin ligase RNF4(RNF4 antibody specific to E3 ubiquitin ligase RNF4(RNF4 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for E3 ubiquitin ligase RNF4(RNF4 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain E3 ubiquitin ligase RNF4(RNF4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of E3 ubiquitin ligase RNF4(RNF4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Parkinsons Disease: Assays for the Ubiquitin Ligase Activity of Neural Parkin
ubiquitin protein ligase activity to wild-type (but not mutant) Parkin proteins. Although the individual components of the proposed Parkin ubiquitin ligase complex in the normal human brain remain to be identified and the E3 ligase effect of Parkin function
Studies of the Ubiquitin Proteasome System
Assays of E1 and E2 Activity Basic Protocol 1: Thiolester Formation Between Rabbit E1 and Ubiquitin Basic Protocol 2: Thiolester Formation Between E2 and Ubiquitin Binding of Ubiquitin‐Proteasome Proteins Basic Protocol 3: Binding of E2s to E3s Alternate
In Vitro Ubiquitination of Cytokine Signaling Components
The eight SOCS (Suppressor of Cytokine Signaling) proteins encoded in the human genome all contain a C-terminal domain, the SOCS box, that allows them to function as E3 ubiquitin ligases and thereby catalyze the ubiquitination of components
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