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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSMBIO
- 检测范围:
0.312-20ng/ml
- 检测方法:
夹心法
- 应用:
夹心法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种:
人
- 标记物:
Human Ubiquitin-conjugating enzyme E2 Z,UBE2Z
- 样本:
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
0.1ng/ml
- 规格:
96T
Human Ubiquitin- conjugating enzyme E2 Z, UBE2Z ELISA KIT
Product Name:Human Ubiquitin- conjugating enzyme E2 Z, UBE2Z ELISA KIT
Packing:96T
Catalog No.:ELI-39970h
Gene Name:UBE2Z
Detect Range:0.312-20 ng/mL
Sensitivity:0.039ng/mL
Target Protein Name:Ubiquitin-conjugating enzyme E2 Z(UBE2Z
Alternative Name:UBE2Z,Ubiquitin-conjugating enzyme E2 Z(UBE2Z
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Ubiquitin- conjugating enzyme E2 Z, UBE2Z ELISA KIT allows for the in vitro quantitative determination of Ubiquitin-conjugating enzyme E2 Z(UBE2Z concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Ubiquitin- conjugating enzyme E2 Z, UBE2Z ELISA KIT has been pre-coated with an Ubiquitin-conjugating enzyme E2 Z(UBE2Z antibody specific to Ubiquitin-conjugating enzyme E2 Z(UBE2Z .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Ubiquitin-conjugating enzyme E2 Z(UBE2Z and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Ubiquitin-conjugating enzyme E2 Z(UBE2Z, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Ubiquitin-conjugating enzyme E2 Z(UBE2Z in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Studies of the Ubiquitin Proteasome System
in Insect Cells Using a Baculovirus System Alternate Protocol 11: Preparation of Rabbit E1 Support Protocol 4: Generation of Empty Bacterial Lysates Generation of Ubiquitin‐Conjugating Enzymes (E2s) Basic Protocol 22: Expression of E2s in E. coli
Proteasomes that Lack Endogenous USP14 and are Devoid of UB‐AMC Hydrolysis Activity Support Protocol 2: Purification of Recombinant Human USP14 from E. coli Support Protocol 3: Synthesis and Purification of Ubiquitin
【分享】Human press METHODS IN MOLECULAR BIOLOGY
.Ubiquitin-Proteasome Protocols Edited by Patterson, Cam 2005 302.Handbook of ELISPOT Methods and Protocols Edited by Kalyuzhny, Alexander E. 2005 303.NanoBiotechnology Protocols Edited by Rosenthal, Sandra J 2005 304.Human Retrovirus Protocols Virology
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