Human Uromodulin-like 1,UMODL1

Human Uromodulin-like 1, Umodl1 ELISA Kit

96 Tests

Operating instructions

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

Umodl1,uromodulin like 1,olfactorin

Search name

Human Umodl1 ELISA KIT ,Human uromodulin like 1 ELISA KIT ,Human olfactorin ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Human Uromodulin-like 1concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.

Test principle

The ELISA is based on the competitive binding enzyme immunoassaytechnique. The microtiter plate provided in this kit has been pre-coated with an antibody specific toUromodulin-like 1, During the reaction,Uromodulin-like 1in the sample or standard competes with a fixed amount of biotin-labeledUromodulin-like 1 for sites on a pre-coated Monoclonal antibody specific to Uromodulin-like 1. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Thena TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Uromodulin-like 1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.