Target Protein Name:Melanoma-associated antigen D1(MAGED1
Alternative Name:MAGED1,Melanoma-associated antigen D1(MAGED1 Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit Product Description:Human Melanoma- associated antigen D1, MAGED1 ELISA KIT allows for the in vitro quantitative determination of Melanoma-associated antigen D1(MAGED1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle: The microtiter plate provided in Human Melanoma- associated antigen D1, MAGED1 ELISA KIT has been pre-coated with an Melanoma-associated antigen D1(MAGED1 antibody specific to Melanoma-associated antigen D1(MAGED1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Melanoma-associated antigen D1(MAGED1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Melanoma-associated antigen D1(MAGED1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Melanoma-associated antigen D1(MAGED1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!