- ￥2890
- LSM Bio
- 0.312-20ng/ml
- 0.1ng/ml
- 进口
- ELI-39555h
- 2025年07月05日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSMBIO
- 检测范围：
0.312-20ng/ml
- 检测方法：
夹心法
- 应用：
夹心法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种：
人
- 标记物：
Human NADPH--cytochrome P450 reductase,POR
- 样本：
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
0.1ng/ml
- 规格：
96T
Human NADPH- - cytochrome P450 reductase, POR ELISA KIT
Product Name:Human NADPH- - cytochrome P450 reductase, POR ELISA KIT
Packing:96T
Catalog No.:ELI-39555h
Gene Name:POR
Detect Range:78-5000 pg/mL
Sensitivity:32pg/mL
Target Protein Name:NADPH--cytochrome P450 reductase(POR
Alternative Name:POR,NADPH--cytochrome P450 reductase(POR
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human NADPH- - cytochrome P450 reductase, POR ELISA KIT allows for the in vitro quantitative determination of NADPH--cytochrome P450 reductase(POR concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human NADPH- - cytochrome P450 reductase, POR ELISA KIT has been pre-coated with an NADPH--cytochrome P450 reductase(POR antibody specific to NADPH--cytochrome P450 reductase(POR .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for NADPH--cytochrome P450 reductase(POR and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain NADPH--cytochrome P450 reductase(POR, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of NADPH--cytochrome P450 reductase(POR in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Catalytic Assays for Human Cytochrome P450
Cytochrome P450 (P450) enzymes catalyze the blotransformatlon of a broad range of structurally diverse foreign chemicals and endogenous substances. Many P450 enzymes have been isolated and identified in liver and other tlssues, including
Cytochrome P450 Reconstitution Systems
Human liver microsomes contain multiple forms of cytochrome P450 (CYP or P450) that catalyze oxidation of a number of xenobiotic and endobiotic chemicals. Individual P450 forms have unique, but overlapping, substrate specificities
Establishment of Functional Human Cytochrome P450 Monooxygenase Systems in Escherichia coli
of the results that can be achieved for the expression of human P450s.
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