- ￥2890
- LSM Bio
- 0.156-10ng/ml
- 0.078ng/ml
- 国产(香港)
- ELI-39476h
- 2026年05月21日
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- 文献和实验
- 技术资料
- 库存：
20
- 供应商：
LSMBIO
- 检测范围：
0.156-10ng/ml
- 检测方法：
夹心法
- 应用：
夹心法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种：
人
- 标记物：
Human N-acetylglutamate synthase,mitochondrial,NAGS
- 样本：
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
0.078ng/ml
- 规格：
96T
Human N- acetylglutamate synthase, mitochondrial, NAGS ELISA KIT
Product Name:Human N- acetylglutamate synthase, mitochondrial, NAGS ELISA KIT
Packing:96T
Catalog No.:ELI-39476h
Gene Name:NAGS
Detect Range:78-5000 pg/mL
Sensitivity:32pg/mL
Target Protein Name:N-acetylglutamate synthase(mitochondrial(NAGS
Alternative Name:NAGS,N-acetylglutamate synthase(mitochondrial(NAGS
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human N- acetylglutamate synthase, mitochondrial, NAGS ELISA KIT allows for the in vitro quantitative determination of N-acetylglutamate synthase(mitochondrial(NAGS concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human N- acetylglutamate synthase, mitochondrial, NAGS ELISA KIT has been pre-coated with an N-acetylglutamate synthase(mitochondrial(NAGS antibody specific to N-acetylglutamate synthase(mitochondrial(NAGS .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for N-acetylglutamate synthase(mitochondrial(NAGS and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain N-acetylglutamate synthase(mitochondrial(NAGS, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of N-acetylglutamate synthase(mitochondrial(NAGS in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验人 Neuritin 酶联免疫分析(ELISA) 试剂盒使用说明书 本试剂仅供研究使用 目的：本试剂盒用于测定人血清，血浆及相关液体样本 中Neuritin 的含量。 实验原理： 本试剂盒应用双抗体夹心法测定标本中人 Neuritin 水平。用纯化的 Neuritin 抗体 包被微孔板，制成固相抗体，往包被单抗的微孔中加入 Neuritin ，再与 HRP 标记的 Neuritin 抗体结合，形成抗体
调至 1μg/μl。 4. ELISA 检测：按试剂盒说明书完成板平衡、加样孵育、洗板、酶标抗体孵育、显色终止与读数，结合标准曲线得出 IFN-γ 浓度。 四、操作要点速记 1.组织样本新鲜处理，全程低温操作，裂解液现配现用； 2. 蛋白定量时标准品与样本同步孵育，稀释采用倍比稀释减少误差； 3. ELISA 实验需每次重绘标准曲线，洗板充分、严格把控显色与终止时间。 免疫检测试剂盒： 货号-规格 名称 abs510001-96T Human IL-2 ELISA Kit (Faster
ELISA原理与实验方法The advantages of the ELISA are similar to other antibody-labeled reactions which include specificity, sensitivity, inexpensiveness, and safety. Since the enzyme label is the critical portion of ELISA, its selection is very important
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