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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSMBIO
- 检测范围:
0.31-20ng/Ml
- 检测方法:
夹心法
- 应用:
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种:
人
- 标记物:
Human NADPH oxidase 3,NOX3
- 样本:
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
0.1ng/Ml
- 规格:
96T
Human NADPH oxidase 3, NOX3 ELISA KIT
Product Name:Human NADPH oxidase 3, NOX3 ELISA KIT
Packing:96T
Catalog No.:ELI-36920h
Gene Name:NOX3
Detect Range:0.156-10 ng/mL
Sensitivity:0.078ng/mL
Target Protein Name:NADPH oxidase 3(NOX3
Alternative Name:NOX3,NADPH oxidase 3(NOX3
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human NADPH oxidase 3, NOX3 ELISA KIT allows for the in vitro quantitative determination of NADPH oxidase 3(NOX3 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human NADPH oxidase 3, NOX3 ELISA KIT has been pre-coated with an NADPH oxidase 3(NOX3 antibody specific to NADPH oxidase 3(NOX3 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for NADPH oxidase 3(NOX3 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain NADPH oxidase 3(NOX3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of NADPH oxidase 3(NOX3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Assessment of the Role for Rho Family GTPases in NADPH Oxidase Activation
Rac, a member of the Rho family small GTPases, plays a crucial role in activation of Nox family NADPH oxidases in animals, enzymes dedicated to production of reactive oxygen species such as superoxide. The phagocyte oxidase Nox2, crucial
Methods for the Detection of D-Amino-Acid Oxidase
al. (5 ) using bovine serum albumin as a standard. It was also determined using a Protein Assay Kit (Bio-Rad, Hercules, CA). D-Amino-acid oxidase activity is finally expressed as the amount of D-alanine oxidized per min per milligram of protein. Hog
The heterodimeric integral membrane protein flavocytochrome b (Cyt b) is the catalytic core of the phagocyte NADPH oxidase, an enzyme complex that initiates a cascade of reactive oxygen species critical for the elimination of infectious
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