- ￥2890
- LSM Bio
- 0.156-10ng/ML
- 0.078 ng/Ml
- 进口
- ELI-35741h
- 2025年07月09日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSMBIO
- 检测范围：
0.156-10ng/ML
- 检测方法：
夹心法
- 应用：
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种：
人
- 标记物：
Human GPI inositol-deacylase,PGAP1
- 样本：
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
0.078 ng/Ml
- 规格：
96T
Human GPI inositol- deacylase, PGAP1 ELISA KIT
Product Name:Human GPI inositol- deacylase, PGAP1 ELISA KIT
Packing:96T
Catalog No.:ELI-35741h
Gene Name:PGAP1
Detect Range:0.312-20 ng/mL
Sensitivity:0.039ng/mL
Target Protein Name:GPI inositol-deacylase(PGAP1
Alternative Name:PGAP1,GPI inositol-deacylase(PGAP1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human GPI inositol- deacylase, PGAP1 ELISA KIT allows for the in vitro quantitative determination of GPI inositol-deacylase(PGAP1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human GPI inositol- deacylase, PGAP1 ELISA KIT has been pre-coated with an GPI inositol-deacylase(PGAP1 antibody specific to GPI inositol-deacylase(PGAP1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for GPI inositol-deacylase(PGAP1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain GPI inositol-deacylase(PGAP1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of GPI inositol-deacylase(PGAP1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验有替代前者的趋势。由于ABS-ELISA较普通ELISA多用了两种试剂，增加了操作步骤，在临床检验中ABS-ELISA应用不多。科研项目中检测微量的成分如细胞因子常采用本法。晶美分装ELISA KIT采用的方法：1， TORCH及传染病试剂盒（间接法），见2.2.32， TORCH-IgM捕获法特色：包被抗体，标记抗原原理：3， 细胞因子试剂盒采用的方法路线（ABC-ELISA）原理产品特色：采用ABC法，灵敏度更高，特异性更强。生物素抗体和酶联物是浓缩的，使用前需用相应的缓冲液稀释。酶联物可以通用
的原理用于分子诊断方面：TrimGen公司的KRAS Mutation Detection Kit在96孔板里一次能实现7/8个KRAS突变位点的检测。 另外基于相同原理的技术还有PPT-ELISA。这是用来筛截短突变的，相关文章见：1，Rapid screen for truncating ATM mutations by PTT-ELISA . 2，An ELISA-based high throughput protein truncation test
R&D Systems Quantikine® 人高分子量脂联素ELISA试剂盒
浆中HMW 脂联素的水平，而不需要对样品进行蛋白水解的预处理。关于脂联素相关产品的更多信息，请访问我们的网站www.RnDSystems.com/Adipocytokines。人HMW 脂联素水平的测定。利用Quantikine Human HMW Adiponectin/Acrp30ELISA Kit（货号 DHWAD0），我们分析了来自不同BMI 个体的血清中的HMW 脂联素平均水平，包括低BMI（平均BMI = 19.8 ；N=4），高BMI（平均BMI = 32.8 ；N=4）和超高BMI（平均
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