- ￥2890
- LSM Bio
- 0.156-10ng/ML
- 0.078 ng/Ml
- 进口
- ELI-35713h
- 2025年07月14日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSMBIO
- 检测范围：
0.156-10ng/ML
- 检测方法：
夹心法
- 应用：
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种：
人
- 标记物：
Human GPI transamidase component PIG-T,PIGT
- 样本：
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
0.078 ng/Ml
- 规格：
96T
Human GPI transamidase component PIG- T, PIGT ELISA KIT
Product Name:Human GPI transamidase component PIG- T, PIGT ELISA KIT
Packing:96T
Catalog No.:ELI-35713h
Gene Name:PIGT
Detect Range:78-5000 pg/mL
Sensitivity:32pg/mL
Target Protein Name:GPI transamidase component PIG-T(PIGT
Alternative Name:PIGT,GPI transamidase component PIG-T(PIGT
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human GPI transamidase component PIG- T, PIGT ELISA KIT allows for the in vitro quantitative determination of GPI transamidase component PIG-T(PIGT concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human GPI transamidase component PIG- T, PIGT ELISA KIT has been pre-coated with an GPI transamidase component PIG-T(PIGT antibody specific to GPI transamidase component PIG-T(PIGT .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for GPI transamidase component PIG-T(PIGT and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain GPI transamidase component PIG-T(PIGT, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of GPI transamidase component PIG-T(PIGT in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Detecting Low‐Affinity Extracellular Protein Interactions Using Protein Microarrays
) HRP‐conjugated anti‐flag antibody Tyramide signal amplification (TSA) kit (Life Technologies, cat. no. T30953) containing
cells/mL) were incubated with LPS (2.5 μg/mL) in the presence or absence of hydroquinone for 6 hours. Supernatants were assayed for the levels of TNF-α, IL-1β, and IL-6 using a mouse TNF-α ELISA kit (Amersham, Little Chalfont, Buckinghamshire, UK).2
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