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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSMBIO
- 检测范围:
0.156-10ng/ML
- 检测方法:
夹心法
- 应用:
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种:
人
- 标记物:
Human Asc-type amino acid transporter 1,SLC7A10
- 样本:
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
0.078 ng/Ml
- 规格:
96T
Human Asc- type amino acid transporter 1, SLC7A10 ELISA KIT
Product Name:Human Asc- type amino acid transporter 1, SLC7A10 ELISA KIT
Packing:96T
Catalog No.:ELI-35059h
Gene Name:SLC7A10
Detect Range:0.156-10 ng/mL
Sensitivity:0.078ng/mL
Target Protein Name:Asc-type amino acid transporter 1(SLC7A10
Alternative Name:SLC7A10,Asc-type amino acid transporter 1(SLC7A10
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Asc- type amino acid transporter 1, SLC7A10 ELISA KIT allows for the in vitro quantitative determination of Asc-type amino acid transporter 1(SLC7A10 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Asc- type amino acid transporter 1, SLC7A10 ELISA KIT has been pre-coated with an Asc-type amino acid transporter 1(SLC7A10 antibody specific to Asc-type amino acid transporter 1(SLC7A10 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Asc-type amino acid transporter 1(SLC7A10 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Asc-type amino acid transporter 1(SLC7A10, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Asc-type amino acid transporter 1(SLC7A10 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Antibody Production and Immunocytochemical Localization of Amino Acid Transporters
This chapter details the procedural and conceptual issues that underpin the localization of high-affinity amino acid transporters by immunocytochemistry, with particular reference to the mammalian nervous system. Defining a role
Isolated plasma membrane vesicles from human placenta allow transporter-mediated mechanisms across individual plasma membranes to be identified and characterized in vitro. This approach is reliant on isolating each of the trophoblast plasma
Methods for the Detection of D-Amino-Acid Oxidase
al. (5 ) using bovine serum albumin as a standard. It was also determined using a Protein Assay Kit (Bio-Rad, Hercules, CA). D-Amino-acid oxidase activity is finally expressed as the amount of D-alanine oxidized per min per milligram of protein. Hog
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