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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSMBIO
- 检测范围:
0.156-10ng/ML
- 检测方法:
夹心法
- 应用:
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种:
人
- 标记物:
Human C->U-editing enzyme APOBEC-1,APOBEC1
- 样本:
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
0.078 ng/Ml
- 规格:
96T
Human C- >U- editing enzyme APOBEC- 1, APOBEC1 ELISA KIT
Product Name:Human C- >U- editing enzyme APOBEC- 1, APOBEC1 ELISA KIT
Packing:96T
Catalog No.:ELI-34986h
Gene Name:APOBEC1
Detect Range:0.312-20 ng/mL
Sensitivity:0.039ng/mL
Target Protein Name:C->U-editing enzyme APOBEC-1(APOBEC1
Alternative Name:APOBEC1,C->U-editing enzyme APOBEC-1(APOBEC1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human C- >U- editing enzyme APOBEC- 1, APOBEC1 ELISA KIT allows for the in vitro quantitative determination of C->U-editing enzyme APOBEC-1(APOBEC1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human C- >U- editing enzyme APOBEC- 1, APOBEC1 ELISA KIT has been pre-coated with an C->U-editing enzyme APOBEC-1(APOBEC1 antibody specific to C->U-editing enzyme APOBEC-1(APOBEC1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for C->U-editing enzyme APOBEC-1(APOBEC1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain C->U-editing enzyme APOBEC-1(APOBEC1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of C->U-editing enzyme APOBEC-1(APOBEC1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Isolation of an mRNA-Binding Protein Involved in C-to-U Editing
with high affinity. Here we outline a protocol for purifying Apobec-1 complementation factor (ACF), the RNA-binding subunit of the apolipoprotein-B (apo-B) mRNA-editing enzyme. ACF was purified using synthetic wild-type and mutant apo-B RNAs
RAT/MOUSE GROWTH HORMONE ELISA KIT
实验原理 This assay is a Sandwich ELISA based, sequentially, on: 1) capture of rat or mouse Growth Hormone molecules from samples to the wells of a microtiter plate coated by a pre-titered amount of anti-Growth Hormone
Enzyme-Linked ImmunoSorbent Assay (ELISA) for NGF
CalbiochemReferenceWeskamp, G., and Otten, U. (1987). An enzyme-linked immunoassay for nerve growth factor (NGF): A tool for studying regulatory mechanisms involved in ngf production in brain and in peripheral tissues. Journal of Neurochemistry, 48 (6),1779-1786.
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