- ￥2890
- LSM Bio
- 0.156-10ng/ML
- 0.078 ng/Ml
- 进口
- ELI-34910h
- 2025年07月14日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSMBIO
- 检测范围：
0.156-10ng/ML
- 检测方法：
夹心法
- 应用：
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种：
人
- 标记物：
Human Muscarinic acetylcholine receptor M1,CHRM1
- 样本：
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
0.078 ng/Ml
- 规格：
96T
Human Muscarinic acetylcholine receptor M1, CHRM1 ELISA KIT
Product Name:Human Muscarinic acetylcholine receptor M1, CHRM1 ELISA KIT
Packing:96T
Catalog No.:ELI-34910h
Gene Name:CHRM1
Detect Range:78-5000 pg/mL
Sensitivity:32pg/mL
Target Protein Name:Muscarinic acetylcholine receptor M1(CHRM1
Alternative Name:CHRM1,Muscarinic acetylcholine receptor M1(CHRM1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Muscarinic acetylcholine receptor M1, CHRM1 ELISA KIT allows for the in vitro quantitative determination of Muscarinic acetylcholine receptor M1(CHRM1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Muscarinic acetylcholine receptor M1, CHRM1 ELISA KIT has been pre-coated with an Muscarinic acetylcholine receptor M1(CHRM1 antibody specific to Muscarinic acetylcholine receptor M1(CHRM1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Muscarinic acetylcholine receptor M1(CHRM1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Muscarinic acetylcholine receptor M1(CHRM1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Muscarinic acetylcholine receptor M1(CHRM1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Overview of Receptor Allosterism
Figure 1.21.1 Allosterism between a receptor and its G protein: the effects of GTP on the competition between [3 H] N ‐methylscopolamine and the agonist, carbachol, at the muscarinic acetylcholine M2 receptor in rat myocardial membranes. Competition
Figure 9.14.6 Saturation data for the binding of [3 H]NMS to the M3 muscarinic receptor. The mean bound cpm is plotted against the amount of added radioligand (radioligand concentration). Specific binding is the difference between total
DREADDs受体作用原理（Scott M. Sternson & Bryan L. Roth, Annu. Rev. Neurosci., 2014）2. 常见的DREADDs受体（1）Gq-DREADD和hM3Dq：最初的Gq-DREADD即hM3Dq，改造自人毒蕈碱型乙酰胆碱受体（the human muscarinic acetylcholine receptor，mAchRs）亚型M3（也称为hM3）。在正常生理状况下，hM3和乙酰胆碱结合，然后和Gq类G蛋白耦合受体耦合，作用于磷脂酶C、肌醇三磷酸
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