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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSMBIO
- 检测范围:
0.156-10ng/ML
- 检测方法:
夹心法
- 应用:
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种:
人
- 标记物:
Human A-kinase anchor protein 11,AKAP11
- 样本:
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
0.078 ng/Ml
- 规格:
96T
Human A- kinase anchor protein 11, AKAP11 ELISA KIT
Product Name:Human A- kinase anchor protein 11, AKAP11 ELISA KIT
Packing:96T
Catalog No.:ELI-34677h
Gene Name:AKAP11
Detect Range:78-5000 pg/mL
Sensitivity:32pg/mL
Target Protein Name:A-kinase anchor protein 11(AKAP11
Alternative Name:AKAP11,A-kinase anchor protein 11(AKAP11
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human A- kinase anchor protein 11, AKAP11 ELISA KIT allows for the in vitro quantitative determination of A-kinase anchor protein 11(AKAP11 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human A- kinase anchor protein 11, AKAP11 ELISA KIT has been pre-coated with an A-kinase anchor protein 11(AKAP11 antibody specific to A-kinase anchor protein 11(AKAP11 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for A-kinase anchor protein 11(AKAP11 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain A-kinase anchor protein 11(AKAP11, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of A-kinase anchor protein 11(AKAP11 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Detection of DNA-Dependent Protein Kinase in Extracts from Human and Rodent Cells
have been used to assay for DNA-PK activity in extracts from human tissue culture cells (8 –10 ) and normal human cells (11 ). DNA-PK activity is readily detected in extracts from human and monkey cell lines but not in protein extracts from nonprimate cells
Translocation Assays of Protein Kinase C Activation
The protein kinase C (PKC) family members include at least 11 different isoforms that, based on their different requirements for activation, have been divided into three subfamilies, the Ca2+ -dependent (cPKCα, (βI , βII
11 Detection of TGF Type II Receptor Hotspot Mutations: The BAT-RII Assay
anchor and intracellular kinase domain. This RII mutation also occurs in gastric and endometrial tumors (4 ) and may be a hot spot in other tumor types with microsatellite instability as well.
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