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- 文献和实验
- 技术资料
- 库存:
20
- 供应商:
LSMBIO
- 检测范围:
0.31-20ng/Ml
- 检测方法:
夹心法
- 应用:
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种:
人
- 标记物:
Human Arylamine N-acetyltransferase 2,NAT2
- 样本:
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
0.1ng/Ml
- 规格:
96T
Human Arylamine N- acetyltransferase 2, NAT2 ELISA KIT
Product Name:Human Arylamine N- acetyltransferase 2, NAT2 ELISA KIT
Packing:96T
Catalog No.:ELI-34285h
Gene Name:NAT2
Detect Range:0.156-10 ng/mL
Sensitivity:0.078ng/mL
Target Protein Name:Arylamine N-acetyltransferase 2(NAT2
Alternative Name:NAT2,Arylamine N-acetyltransferase 2(NAT2
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Arylamine N- acetyltransferase 2, NAT2 ELISA KIT allows for the in vitro quantitative determination of Arylamine N-acetyltransferase 2(NAT2 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Arylamine N- acetyltransferase 2, NAT2 ELISA KIT has been pre-coated with an Arylamine N-acetyltransferase 2(NAT2 antibody specific to Arylamine N-acetyltransferase 2(NAT2 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Arylamine N-acetyltransferase 2(NAT2 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Arylamine N-acetyltransferase 2(NAT2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Arylamine N-acetyltransferase 2(NAT2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Phenotypic and Genotypic Characterization of N-Acetylation
as the N -acetylation polymorphism. This chapter provides as background a description of some key studies that showed that acetylation was variable in the human population, that it is inherited, and the molecular basis for this phenomenon. Since accurate
人 Neuritin 酶联免疫分析(ELISA) 试剂盒使用说明书 本试剂仅供研究使用 目的:本试剂盒用于测定人血清,血浆及相关液体样本 中Neuritin 的含量。 实验原理: 本试剂盒应用双抗体夹心法测定标本中人 Neuritin 水平。用纯化的 Neuritin 抗体 包被微孔板,制成固相抗体,往包被单抗的微孔中加入 Neuritin ,再与 HRP 标记的 Neuritin 抗体结合,形成抗体
调至 1μg/μl。 4. ELISA 检测:按试剂盒说明书完成板平衡、加样孵育、洗板、酶标抗体孵育、显色终止与读数,结合标准曲线得出 IFN-γ 浓度。 四、操作要点速记 1.组织样本新鲜处理,全程低温操作,裂解液现配现用; 2. 蛋白定量时标准品与样本同步孵育,稀释采用倍比稀释减少误差; 3. ELISA 实验需每次重绘标准曲线,洗板充分、严格把控显色与终止时间。 免疫检测试剂盒: 货号-规格 名称 abs510001-96T Human IL-2 ELISA Kit (Faster
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