Product Name:Human Centromere protein W, CENPW ELISA KIT Packing:96T
Catalog No.:ELI-34209h
Gene Name:CENPW
Detect Range:0.312-20 ng/mL
Sensitivity:0.039ng/mL
Target Protein Name:Centromere protein W(CENPW
Alternative Name:CENPW,Centromere protein W(CENPW Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit Product Description:Human Centromere protein W, CENPW ELISA KIT allows for the in vitro quantitative determination of Centromere protein W(CENPW concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle: The microtiter plate provided in Human Centromere protein W, CENPW ELISA KIT has been pre-coated with an Centromere protein W(CENPW antibody specific to Centromere protein W(CENPW .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Centromere protein W(CENPW and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Centromere protein W(CENPW, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Centromere protein W(CENPW in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!