- ￥2890
- LSM Bio
- 0.156-10ng/ML
- 0.078 ng/Ml
- 进口
- ELI-34203h
- 2025年07月09日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSMBIO
- 检测范围：
0.156-10ng/ML
- 检测方法：
夹心法
- 应用：
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种：
人
- 标记物：
Human Centromere-associated protein E,CENPE
- 样本：
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
0.078 ng/Ml
- 规格：
96T
Human Centromere- associated protein E, CENPE ELISA KIT
Product Name:Human Centromere- associated protein E, CENPE ELISA KIT
Packing:96T
Catalog No.:ELI-34203h
Gene Name:CENPE
Detect Range:0.156-10 ng/mL
Sensitivity:0.078ng/mL
Target Protein Name:Centromere-associated protein E(CENPE
Alternative Name:CENPE,Centromere-associated protein E(CENPE
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Centromere- associated protein E, CENPE ELISA KIT allows for the in vitro quantitative determination of Centromere-associated protein E(CENPE concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Centromere- associated protein E, CENPE ELISA KIT has been pre-coated with an Centromere-associated protein E(CENPE antibody specific to Centromere-associated protein E(CENPE .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Centromere-associated protein E(CENPE and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Centromere-associated protein E(CENPE, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Centromere-associated protein E(CENPE in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Medium-Throughput Production of Recombinant Human Proteins: Protein Production in E. coli
In Chapter 4 we described the SGC process for generating multiple constructs of truncated versions of each protein using LIC. In this chapter we provide a step-by-step procedure of our E . coli system for test expressing intracellular
E.Z.N.A.® Endo-free Plasmid Mini Kit I Spin Protocol
as above. This step ensures that residual protein contamination is removed and must be included for downstream application requiring high quality DNA. 12. Discard the flow-through liquid and wash the column by adding 700 u DNA Wash Buffer diluted with ethanol
E.Z.N.A. Endo-Free Plasmid Mini Kit II Spin Protocol
through the column. 11. Wash column with 500 ul Buffer HB and centrifuge as above. This step ensures that residual protein contamination is removed and must be included for downstream application requiring high quality DNA. 12. Discard the flow
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