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- 文献和实验
- 技术资料
- 库存:
20
- 供应商:
LSMBIO
- 检测范围:
0.156-10ng/ml
- 检测方法:
夹心法/竞争法
- 应用:
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种:
人
- 标记物:
Human Bcl-2-interacting killer,BIK
- 样本:
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
0.078ng/ml
- 规格:
96T
Human Bcl- 2- interacting killer, BIK ELISA KIT
Product Name:Human Bcl- 2- interacting killer, BIK ELISA KIT
Packing:96T
Catalog No.:ELI-33332h
Gene Name:BIK
Detect Range:0.312-20 ng/mL
Sensitivity:0.039ng/mL
Target Protein Name:Bcl-2-interacting killer(BIK
Alternative Name:BIK,Bcl-2-interacting killer(BIK
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Bcl- 2- interacting killer, BIK ELISA KIT allows for the in vitro quantitative determination of Bcl-2-interacting killer(BIK concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Bcl- 2- interacting killer, BIK ELISA KIT has been pre-coated with an Bcl-2-interacting killer(BIK antibody specific to Bcl-2-interacting killer(BIK .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Bcl-2-interacting killer(BIK and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Bcl-2-interacting killer(BIK, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Bcl-2-interacting killer(BIK in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Gene-Specific PCR Typing of Killer Cell Immunoglobulin-Like Receptors
By interacting with specific HLA class I molecules, the killer cell immunoglobulin-like receptors (KIR) regulate the effector function of natural killer (NK) cells and subsets of CD8 T cells. The KIR receptors and HLA class I ligands
调至 1μg/μl。 4. ELISA 检测:按试剂盒说明书完成板平衡、加样孵育、洗板、酶标抗体孵育、显色终止与读数,结合标准曲线得出 IFN-γ 浓度。 四、操作要点速记 1.组织样本新鲜处理,全程低温操作,裂解液现配现用; 2. 蛋白定量时标准品与样本同步孵育,稀释采用倍比稀释减少误差; 3. ELISA 实验需每次重绘标准曲线,洗板充分、严格把控显色与终止时间。 免疫检测试剂盒: 货号-规格 名称 abs510001-96T Human IL-2 ELISA Kit (Faster
Human IL-2 Enzyme Immunoassay (EIA)
-2 EIA is an in vitro enzyme-linked immunosorbent assay for quantitatively detecting human IL-2 in biological fluids (e.g. serum, plasma) and in cell culture supernatants. The kit consists of a 96 well microtiter plate that has been precoated with anti-IL
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