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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSMBIO
- 检测范围:
0.156-10ng/ml
- 检测方法:
夹心法/竞争法
- 应用:
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种:
人
- 标记物:
Human D-amino acid oxidase activator,DAOA
- 样本:
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
0.078ng/ml
- 规格:
96T
Human D- amino acid oxidase activator, DAOA ELISA KIT
Product Name:Human D- amino acid oxidase activator, DAOA ELISA KIT
Packing:96T
Catalog No.:ELI-32379h
Gene Name:DAOA
Detect Range:0.156-10 ng/mL
Sensitivity:0.078ng/mL
Target Protein Name:D-amino acid oxidase activator(DAOA
Alternative Name:DAOA,D-amino acid oxidase activator(DAOA
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human D- amino acid oxidase activator, DAOA ELISA KIT allows for the in vitro quantitative determination of D-amino acid oxidase activator(DAOA concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human D- amino acid oxidase activator, DAOA ELISA KIT has been pre-coated with an D-amino acid oxidase activator(DAOA antibody specific to D-amino acid oxidase activator(DAOA .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for D-amino acid oxidase activator(DAOA and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain D-amino acid oxidase activator(DAOA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of D-amino acid oxidase activator(DAOA in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Methods for the Detection of D-Amino-Acid Oxidase
al. (5 ) using bovine serum albumin as a standard. It was also determined using a Protein Assay Kit (Bio-Rad, Hercules, CA). D-Amino-acid oxidase activity is finally expressed as the amount of D-alanine oxidized per min per milligram of protein. Hog
Methods for the Detection of D-Amino-Acid Oxidase
al. ( 5 ) using bovine serum albumin as a standard. It was also determined using a Protein Assay Kit (Bio-Rad, Hercules, CA). D-Amino-acid oxidase activity is finally expressed as the amount of D-alanine oxidized per min per milligram of protein. Hog
transcription activator?like effector nucleases (TALENs) are broadly useful tools for performing targeted genome editing in a wide variety of organisms and cell types including plants, zebrafish, C. elegans , rat, human somatic cells, and human pluripotent stem
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