- ￥2890
- LSM Bio
- 0.156-10ng/ml
- 0.078ng/ml
- 国产
- ELI-31932h
- 2025年07月13日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSMBIO
- 检测范围：
0.156-10ng/ml
- 检测方法：
夹心法/竞争法
- 应用：
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种：
人
- 标记物：
Human Cytochrome P450 1A2,CYP1A2
- 样本：
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
0.078ng/ml
- 规格：
96T
Human Cytochrome P450 1A2, Cyp1a2 ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
Class 1.14.14.1,Cytochrome P450 1A2,Cytochrome P450-D,Cytochrome P-450d,CYPIA2,Cytochrome P-448, CYP1A2,cytochrome P450 1A2; CP12; P3-450; P450(PA); CYPIA2; P450 form 4; aryl hydrocarbon hydroxylase; cytochrome P(3)450; cytochrome P450 4; cytochrome P450, subfamily I (aromatic compound-inducible), polypeptide 2; cytochrome P450-P3; dioxin-inducible P3-450; flavoprotein-linked monooxygenase; microsomal monooxygenase; xenobiotic monooxygenase; cytochrome P450 family 1 subfamily A polypeptide 2
Search name
Human Class 1.14.14.1 ELISA KIT ,Human Cytochrome P450 1A2 ELISA KIT ,Human Cytochrome P450-D ELISA KIT ,Human Cytochrome P-450d ELISA KIT ,Human CYPIA2 ELISA KIT ,Human Cytochrome P-448 ELISA KIT ,Human CYP1A2 ELISA KIT ,Human cytochrome P450 1A2 ELISA KIT ,Human CP12 ELISA KIT ,Human P3-450 ELISA KIT ,Human P450(PA) ELISA KIT ,Human CYPIA2 ELISA KIT ,Human P450 form 4 ELISA KIT ,Human aryl hydrocarbon hydroxylase ELISA KIT ,Human cytochrome P(3)450 ELISA KIT ,Human cytochrome P450 4 ELISA KIT ,Human cytochrome P450 ELISA KIT ,Human subfamily I (aromatic compound-inducible) ELISA KIT ,Human polypeptide 2 ELISA KIT ,Human cytochrome P450-P3 ELISA KIT ,Human dioxin-inducible P3-450 ELISA KIT ,Human flavoprotein-linked monooxygenase ELISA KIT ,Human microsomal monooxygenase ELISA KIT ,Human xenobiotic monooxygenase ELISA KIT ,Human cytochrome P450 family 1 subfamily A polypeptide 2 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Human Cytochrome P450 1A2 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cytochrome P450 1A2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Cytochrome P450 1A2 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Cytochrome P450 1A2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Cytochrome P450 1A2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验Evaluation of Cytochrome P450 Activities in Human Hepatocytes In Vitro
Major hepatic cytochrome P450 activities (CYP1A2, CYP2A6, CYP2B6, CYP2C9, CYP2C19, CYP2D6, CYP2E1, and CYP3A4) can be simultaneously examined in human hepatocytes by incubation with a cocktail of multiple specific probes. Cocktail strategy
Cytochrome P450 Reconstitution Systems
Human liver microsomes contain multiple forms of cytochrome P450 (CYP or P450) that catalyze oxidation of a number of xenobiotic and endobiotic chemicals. Individual P450 forms have unique, but overlapping, substrate specificities
Evaluation of Cytochrome P450 Inhibition in Human Liver Microsomes
Evaluation of lead compounds for P450 inhibition in human liver microsomes has been widely accepted as an in vitro approach to assess drug interaction potential. This chapter describes a detailed traditional CYP inhibition protocol
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