- ￥2890
- LSM Bio
- 0.156-10ng/ml
- 0.078ng/ml
- 国产
- ELI-31343h
- 2025年07月14日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSMBIO
- 检测范围：
0.156-10ng/ml
- 检测方法：
夹心法/竞争法
- 应用：
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种：
人
- 标记物：
Human Transmembrane glycoprotein NMB,GPNMB
- 样本：
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
0.078ng/ml
- 规格：
96T
Human Transmembrane glycoprotein NMB, GPNMB ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
GPNMB,HGFIN; NMB; glycoprotein (transmembrane) nmb; glycoprotein nmb-like protein; glycoprotein nonmetastatic melanoma protein B; hematopoietic growth factor inducible neurokinin-1; osteoactivin; transmembrane glycoprotein HGFIN; glycoprotein nmb, Transmembrane glycoprotein NMB,Osteoactivin,DC-HIL,Dendritic cell-associated transmembrane protein
Search name
Human GPNMB ELISA KIT ,Human HGFIN ELISA KIT ,Human NMB ELISA KIT ,Human glycoprotein (transmembrane) nmb ELISA KIT ,Human glycoprotein nmb-like protein ELISA KIT ,Human glycoprotein nonmetastatic melanoma protein B ELISA KIT ,Human hematopoietic growth factor inducible neurokinin-1 ELISA KIT ,Human osteoactivin ELISA KIT ,Human transmembrane glycoprotein HGFIN ELISA KIT ,Human glycoprotein nmb ELISA KIT ,Human Transmembrane glycoprotein NMB ELISA KIT ,Human Osteoactivin ELISA KIT ,Human DC-HIL ELISA KIT ,Human Dendritic cell-associated transmembrane protein ELISA KIT
Intended use
Human GPNMB ELISA KIT allows for the in vitro quantitative determination of human transmembrane glycoprotein nmb,Transmembrane glycoprotein NMB concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in Human GPNMB ELISA KIT has been pre-coated with an antibody specific to Transmembrane glycoprotein NMB. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Transmembrane glycoprotein NMB and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Transmembrane glycoprotein NMB, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Transmembrane glycoprotein NMB in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验PriCells: Introduction of Isolation and Culture of Human Alveolar Epithelial Cells
markers, specific for type I cells, have been described in the recent past. The first one was T1α, a 36-kDa glycoprotein found in rodent lungs. Antibodies have been developed against lung proteins that specifically label type I cells in human lung
【分享】Human press METHODS IN MOLECULAR BIOLOGY
Protocols Volume II: Differentiation Models Edited by Turksen, Kursad 2006 331. Human Embryonic Stem Cell Protocols Edited by Turksen, Kursad 2006 332. Transmembrane Signaling Protocols 2nd Edited by Ali, Hydar 2006 333. Transplantation Immunology
有替代前者的趋势。由于ABS-ELISA较普通ELISA多用了两种试剂，增加了操作步骤，在临床检验中ABS-ELISA应用不多。科研项目中检测微量的成分如细胞因子常采用本法。晶美分装ELISA KIT采用的方法：1， TORCH及传染病试剂盒（间接法），见2.2.32， TORCH-IgM捕获法特色：包被抗体，标记抗原原理：3， 细胞因子试剂盒采用的方法路线（ABC-ELISA）原理产品特色：采用ABC法，灵敏度更高，特异性更强。生物素抗体和酶联物是浓缩的，使用前需用相应的缓冲液稀释。酶联物可以通用
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