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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSMBIO
- 检测范围:
0.156-10ng/ml
- 检测方法:
夹心法/竞争法
- 应用:
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种:
人
- 标记物:
Human Kinesin-like protein KIF22,KIF22
- 样本:
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
0.078ng/ml
- 规格:
96T
Human Kinesin- like protein KIF22, KIF22 ELISA KIT
Product Name:Human Kinesin- like protein KIF22, KIF22 ELISA KIT
Packing:96T
Catalog No.:ELI-31169h
Gene Name:KIF22
Detect Range:0.312-20 ng/mL
Sensitivity:0.039ng/mL
Target Protein Name:Kinesin-like protein KIF22(KIF22
Alternative Name:KIF22,Kinesin-like protein KIF22(KIF22
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Kinesin- like protein KIF22, KIF22 ELISA KIT allows for the in vitro quantitative determination of Kinesin-like protein KIF22(KIF22 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Kinesin- like protein KIF22, KIF22 ELISA KIT has been pre-coated with an Kinesin-like protein KIF22(KIF22 antibody specific to Kinesin-like protein KIF22(KIF22 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Kinesin-like protein KIF22(KIF22 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Kinesin-like protein KIF22(KIF22, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Kinesin-like protein KIF22(KIF22 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Crystallization of Kinesin Family Motor Proteins
Motor proteins of several kinesin family groups have now been crystallized: monomeric Kinesin-1 motor domains from human,rat and Neurospora (Kull et al.,1996; Sack et al.,1997; Song et al.,2001)and dimeric rat Kinesin-1 (two motor domains connected
Crystallization of Kinesin Family Motor Proteins
Construct Method Conditions References Human Kinesin-1 hK349 Sitting drop 4°C 5 mg/ml protein in 50 mM Na-acetate, pH 4.6, 75 mM KCl, 3.5% (w/v) PEG 4000
technical steps to recover uPA and PAI-1 protein from archived breast cancer tissues and to quantitatively determine uPA and PAI-1 protein content in tumor tissue extracts by enzyme-linked immunosorbent assay (ELISA) are described in detail. The technical
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